We narrowed to 8,725 results for: Set

1. pDD285

   Plasmid

   #66826

   Purpose

   mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   mKate2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized mKate2

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-CWB-EGFP

   Plasmid

   #61462

   Purpose

   AAV vector with CaMKIIa promoter, EGFP, WPRE, and bGHpA

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CaMKIIa

   Available Since

   Feb. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAbaMCi-GFP-BsaI_pJMP2748

   Plasmid

   #208888

   Purpose

   Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette, sfGFP
   

   Use

   Pir dependent origin of replication

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD363

   Plasmid

   #91829

   Purpose

   LoxP-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   LoxP-SEC-LoxP
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXF276

   Plasmid

   #139048

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   lov domain (includes STOP codon)
   

   Expression

   Worm

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSDH52

   Plasmid

   #139049

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   epdz (includes STOP codon)
   

   Expression

   Worm

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNM3814

   Plasmid

   #154122

   Purpose

   mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   mec-4p::rtetR-L-QFAD::act-4 '3
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDD364

    Plasmid

    #91831

    Purpose

    Lox511I-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox511I-SEC-Lox511I
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD382

    Plasmid

    #91830

    Purpose

    Lox2272-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox2272-SEC-Lox2272
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRKO

    Plasmid

    #49456

    Purpose

    loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

    Depositor

    Marc Gartenberg

    Article

    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

    Insert

    loxP-kanMX-loxP cassette
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    none

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Launchpad (AVA2590)

    Plasmid

    #85442

    Purpose

    Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    FRT-flanked EF1alfa-GFP PGK-Puromycin cassette
    

    Use

    Lentiviral

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pMYO127TAG-SepT

    Plasmid

    #34625

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin-127TAG
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized + an amber stop codon was introdu…

    Promoter

    Ipp

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCM935

    Plasmid

    #58202

    Purpose

    Expresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    unc-22 sgRNA
    

    Expression

    Worm

    Promoter

    U6

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pSDH61

    Plasmid

    #139033

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (no STOP codon)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe puro MTH1

    Plasmid

    #21295

    Depositor

    Bob Weinberg

    Article

    Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    MTH1 (NUDT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pGGF012

    Plasmid

    #48849

    Purpose

    Provides a sulfadiazine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pMAS::SulfadiazineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition sites in SulfR removed …

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDM1079

    Plasmid

    #108979

    Purpose

    Knockout cassette Blasticidin

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    Knockout cassette Blasticidin
    

    Use

    Cre/Lox; Dictyostelium discoideum knockout vector

    Promoter

    coaA

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPL5628_TEF1*-Cre_MET15

    Plasmid

    #60933

    Purpose

    Constitutive expression vector for Cre-recombinase containing MET15 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Promoter

    mutant TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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