Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,974 results for: codon optimized

Clear keywords
Showing: 2181 - 2200 of 4974 results

  3. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid
    #70656
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against DDX3Y
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Publication
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Promoter
    sp6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

    Enlarge

  6. EcSTH

    Plasmid
    #211923
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which can be used to increase NADH/NAD ratio and decrease NADPH/NADP ratio in mammalian cells.
    Depositor
    Binghui Li
    Publication
    Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xFLAG
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211923-plasmid-map-sequence-id-429006

    Enlarge

  7. pPMVAugw

    Plasmid
    #98300
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.
    Depositor
    Claudia Vickers
    Publication
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    TEFM1EfmvaE>TEBS1
    Expression
    Yeast
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98300-plasmid-map-sequence-id-190179

    Enlarge

  8. pcDNA3.1(-)-V5.hOr47a

    Plasmid
    #126474
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Publication
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Or47a (Or47a Fly)
    Tags
    V5 tag
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    126474-plasmid-map-sequence-id-254718

    Enlarge

  9. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid
    #92353
    Purpose
    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFlag-NLS-wild-type SpCas9-NLS
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92353-plasmid-map-sequence-id-178243

    Enlarge

  10. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

    Enlarge

  11. pIA033

    Plasmid
    #200420
    Purpose
    thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.
    Depositor
    Rut Carballido-López
    Publication
    Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016
    Type
    Empty backbone
    Tags
    bsPAmCherry
    Expression
    Bacterial
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200420-plasmid-map-sequence-id-403933

    Enlarge

  13. pCS2+/C-Halo

    Plasmid
    #184418
    Purpose
    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Publication
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    Halo-tag
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184418-plasmid-map-sequence-id-373502

    Enlarge

  14. pCS2+/N-SNAPf

    Plasmid
    #184415
    Purpose
    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Publication
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    SNAPf-tag
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184415-plasmid-map-sequence-id-365684

    Enlarge

  19. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid
    #70658
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. lentiCRISPR - Amplicon, JAK2 sgRNA 1

    Plasmid
    #70679
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2181 - 2200 of 4974 results