We narrowed to 11,674 results for: pcas

1. pCAG roGFP2-mito

   Plasmid

   #168509

   Purpose

   Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-roGFP2
   

   Tags

   roGFP2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG roGFP2-mito

   Plasmid

   #168509

   Purpose

   Expression of mito-roGFP2 (mitochondrial matrix targeted roGFP2) used for monitoring dynamcs of mitochondrial Redox potential

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-roGFP2
   

   Tags

   roGFP2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-OSF-KATNA1

   Plasmid

   #160051

   Purpose

   Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Tags

   One-Strep-Flag (OSF)

   Expression

   Mammalian

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-OSF-KATNA1

   Plasmid

   #160051

   Purpose

   Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Tags

   One-Strep-Flag (OSF)

   Expression

   Mammalian

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG2-EGFP-C-IFT140

   Plasmid

   #218739

   Purpose

   Expresses C-terminally EGFP-tagged IFT140 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT140 (IFT140 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG2-EGFP-C-IFT140

   Plasmid

   #218739

   Purpose

   Expresses C-terminally EGFP-tagged IFT140 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Insert

   IFT140 (IFT140 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-HF1-plus

   Plasmid

   #126768

   Purpose

   Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SpCas9-HF1-plus

   Plasmid

   #126768

   Purpose

   Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-SpCas9-HF1

   Plasmid

   #108301

   Purpose

   pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-SpCas9-HF1

    Plasmid

    #108301

    Purpose

    pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG_Xph20-mEos3.2-CCR5TC

    Plasmid

    #135532

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG_Xph20-mEos3.2-CCR5TC

    Plasmid

    #135532

    Purpose

    Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph20
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-sp-flag-hSema4D

    Plasmid

    #190656

    Purpose

    Expresses Human Sema4D with N terminal flag after signal peptide

    Depositor

    Andrew Newman, Victor Tarabykin

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4D (SEMA4D Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-sp-flag-hSema4D

    Plasmid

    #190656

    Purpose

    Expresses Human Sema4D with N terminal flag after signal peptide

    Depositor

    Andrew Newman, Victor Tarabykin

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4D (SEMA4D Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-eNpHR3.0-mRuby2-ST

    Plasmid

    #109134

    Purpose

    Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eNpHR3.0-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-eNpHR3.0-mRuby2-ST

    Plasmid

    #109134

    Purpose

    Halorhodopsin fused to mRuby2 and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eNpHR3.0-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-R2Tg-3xFlag

    Plasmid

    #223250

    Purpose

    Mammalian expression of C-terminally Flag-tagged R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-R2Tg-3xFlag

    Plasmid

    #223250

    Purpose

    Mammalian expression of C-terminally Flag-tagged R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-5HA-mISG15

    Plasmid

    #12444

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15
    

    Tags

    5xHA

    Expression

    Mammalian

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pCAGGS-5HA-mISG15

    Plasmid

    #12444

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15
    

    Tags

    5xHA

    Expression

    Mammalian

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only