We narrowed to 5,339 results for: 290

1. RhoC gRNA #1

   Plasmid

   #198724

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #1

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RhoC gRNA #2

   Plasmid

   #198725

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #2

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. RhoC gRNA #3

   Plasmid

   #198726

   Purpose

   Cas9-mediated knockout of RhoC in mammalian cells #3

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   RhoC (Rhoc Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiCRISPRv2 EGFP sgRNA4

   Plasmid

   #164934

   Purpose

   Expresses EGFP sgRNA4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2 NT sgRNA5

   Plasmid

   #164930

   Purpose

   Expresses Non-targeting sgRNA5 control in mammalian cells

   Depositor

   Ji Luo

   Article

   Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

   Insert

   Non-targeting sgRNA5 (verified for knockout)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRH3175

   Plasmid

   #210177

   Purpose

   Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1

   Depositor

   Randy Hampton

   Article

   Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

   Insert

   YIp ADE2-LEU2 pTDH3::aro7-R33G,G141S-GFP::tADH1 (ARO7 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R33G,G141S

   Promoter

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMx/ATP10A(E203Q)-HA

   Plasmid

   #209258

   Purpose

   Mammalian expression of ATP10A mutant E203Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10A (ATP10A Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   E203Q

   Promoter

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG/ATP10A(E203Q)-HA

   Plasmid

   #209246

   Purpose

   Mammalian expression of ATP10A mutant E203Q

   Depositor

   Hye-Won Shin

   Article

   Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

   Insert

   ATP10A (ATP10A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   E203Q

   Promoter

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFsynW SYT1-PGM reporter

    Plasmid

    #197278

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Phat2-dr-Alox5-PLAT-EGFP-6H

    Plasmid

    #187189

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000057273 (alox5a Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFlare9A-CD45i4e5i5

    Plasmid

    #131834

    Purpose

    A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    PTPRC (PTPRC Human)
    

    Use

    Reporter construct

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV V5-mRnf185

    Plasmid

    #175126

    Purpose

    Lentiviral expression of V5-tagged mouse Rnf185

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Rnf185 (Rnf185 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV Tmem43-V5

    Plasmid

    #175111

    Purpose

    Lentiviral expression of mouse Tmem43-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Tmem43 (Tmem43 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 LIC 6A CARD8-FLAG F405G

    Plasmid

    #169956

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Grin1 gRNA#3

    Plasmid

    #169791

    Purpose

    Cas9-mediated knockout of Grin1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Grin1 gRNA#2

    Plasmid

    #169790

    Purpose

    Cas9-mediated knockout of Grin1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBtPIPLC_N168C His

    Plasmid

    #173809

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Mary F Roberts, Anne Gershenson

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Asn168Cys for labeling

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Grin1 gRNA#1

    Plasmid

    #169789

    Purpose

    Cas9-mediated knockout of Grin1 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221 CARD8 FIIND L370G

    Plasmid

    #169970

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    L370G

    Promoter

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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