We narrowed to 5,083 results for: mcs

1. mAMCase_CatD_6xHis_pcDNA3.1

   Plasmid

   #200229

   Purpose

   Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

   Depositor

   James S Fraser

   Article

   Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

   Insert

   Acidic Mammalian Chitinase (Chia1 Mouse)
   

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S354F

   Promoter

   CMV

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCENPBR pc861

   Plasmid

   #167569

   Purpose

   Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   Homo sapiens centromere protein B (CENPB Human)
   

   Tags

   Ds-Red

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3_HTR2A-NTEV-TCS-GV-2xHA

   Plasmid

   #194366

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   HTR2A-NTEV-TCS-GV-2xHA (HTR2A Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3_SP-DRD1-NTEV-TCS-GV-2xHA

   Plasmid

   #194359

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-DRD1-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3_DRD1-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194360

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3_DRD2-NTEV-TCS-GV-2xHA

   Plasmid

   #194362

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   DRD2-NTEV-TCS-GV-2xHA (DRD2 Human, Synthetic)
   

   Tags

   2xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pODN-H2BC11-TagBFP

   Plasmid

   #183867

   Purpose

   Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mTagBFP

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-mR2-ECT2_sgRNA

   Plasmid

   #183875

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-43

   Plasmid

   #172759

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-43
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-uTEV1-MAVS-mTA3

    Plasmid

    #171001

    Purpose

    HiLITR protease with MAVS [Y536delinsFIV] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIV]
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[Y536delinsFIV]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-35

    Plasmid

    #172755

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-35
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaG-19

    Plasmid

    #172748

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-19
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21-pelB-LaG-3

    Plasmid

    #172740

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET21-pelB-LaG-12

    Plasmid

    #172744

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-12
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-pelB-LaG-14

    Plasmid

    #172745

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-14
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL1-neoR

    Plasmid

    #117048

    Purpose

    In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    neoR
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP64-hERG1a D540K

    Plasmid

    #53057

    Purpose

    Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Mutation

    changed Aspartate 540 to Lysine

    Promoter

    SP6

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSP64-hERG1a S620T

    Plasmid

    #53059

    Purpose

    Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Mutation

    changed Serine 620 to Threonine

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSP64-hERG1a S631A

    Plasmid

    #53058

    Purpose

    Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Mutation

    changed Serine 631 to Alanine

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLM24 ∆lpxM

    Bacterial Strain

    #132780

    Purpose

    CLM24 strain with lpxM deletion

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Bacterial Resistance

    None

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only