We narrowed to 9,006 results for: CAG

1. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-MBP-Foldon-ATG9 (692-839)

   Plasmid

   #188087

   Purpose

   To make ATG9 C-terminal tail trimer for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Use

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   ATG9 (692-839)

   Promoter

   CMV

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108837

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108841

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   PYL

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA

   Plasmid

   #108847

   Purpose

   Insert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

   Plasmid

   #133788

   Purpose

   CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized St3Cas9
   

   Use

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-FNLS-T2A-EGFP-ires-puro

   Plasmid

   #121169

   Purpose

   CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.

   Depositor

   Timo Otonkoski

   Insert

   FNLS-T2A-EGFP-ires-puro
   

   Use

   CRISPR

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-WIPI2d10-364delta263-295-TEV-STREP

   Plasmid

   #171830

   Purpose

   Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   10-364delta263-295

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-FLAG-IRES-blasticidin-Sox2 S248A

   Plasmid

   #182047

   Purpose

   expression of Sox2 mutants

   Depositor

   Hyonchol Jang

   Article

   Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S248A

   Promoter

   CAG

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-FLAG-IRES-blasticidin-Sox2 T258A

    Plasmid

    #182048

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T258A

    Promoter

    CAG

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-CAG-mNanog-mut-pgk-hph

    Plasmid

    #74914

    Purpose

    Mammalian expression of mNanog (Y42E, K43T)

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K43T of Nanog protein and Y42E of Nanog homeodoma…

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid

    #133790

    Purpose

    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized NmeCas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXL-BacII-CAG-Zeo-t2A-sHRPaNRX3beta

    Plasmid

    #79913

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPaNRX3beta

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-sHRPbNRX3beta
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA

    Plasmid

    #108839

    Purpose

    Insert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid

    #121171

    Purpose

    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    xFNLS-T2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpA

    Plasmid

    #108824

    Purpose

    Expresses aa271 to C-terminus of Cre recombinase fused to pMag CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment aa271 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and pMag

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1

    Plasmid

    #79914

    Purpose

    piggyBac transposon vector, Zeocin-2A-sHRPbNLG1

    Depositor

    Joshua Sanes

    Article

    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

    Insert

    Zeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpA

    Plasmid

    #108802

    Purpose

    Expresses aa429 to C-terminus of PhiC31 integrase fused to PYL CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa429 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS and PYL

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

    Plasmid

    #87586

    Purpose

    PV7 for 3-input, 2-output Full Adder

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAG-loxP-3xpA-sfGFP-tdTomato T2A V XE

    Plasmid

    #113853

    Purpose

    Superfolder GFP and tdTomato linked by T2A (V linker)

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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