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pUCBB-pBAD-eGFP

Plasmid

#32553

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

pBAD

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pShuttle-FEN1DA

Plasmid

#35028

Depositor

Sheila Stewart

Publication

Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

Insert

FEN1DA (FEN1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

D181A--NUCLEASE DEAD POINT MUTANT

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Publication

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAC1812_pCR8-gCasRx-CUG (control gRNA)

Plasmid

#118645

Purpose

Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gCasRx-CUG

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX317-RBFOX1

Plasmid

#115442

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

RBFOX1 (RBFOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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hCXCR4-LSSmOrange

Plasmid

#110197

Purpose

Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

Depositor

Sergi Padilla Parra

Publication

Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hCXCR4 (CXCR4 Human)

Tags

LSSmOrange

Expression

Mammalian

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YCpLac111

Plasmid

#53249

Purpose

contains a single replication origin (ARS1) used for plasmid replication assay

Depositor

Jean Cook

Publication

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Type

Empty backbone

Use

Unspecified

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCRU5-inmCherry

Plasmid

#60238

Purpose

HTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Publication

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inmCherry

Use

Retroviral

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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GST-PBD

Plasmid

#60880

Purpose

Bacterially expressed GST-tagged human polo-like kinase 1 PBD AA365-653

Depositor

Trina Schroer

Publication

Yeh et al EMBO J. 2013 Apr 3;32(7):1023-35. doi: 1

Insert

Polo-like kinase 1 (PLK1 BC01486, Human)

Tags

GST

Expression

Bacterial

Available since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAGGS SARS-CoV-2 BA.2 Spike

Plasmid

#185604

Purpose

Expresses codon-optimized full length SARS-CoV-2 BA.2 Spike

Depositor

Marceline Côté

Publication

Tauzin et al Cell Rep. 2022 Oct 25;41(4):111554. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 BA.2 Spike (S )

Expression

Mammalian

Mutation

T19I, L24S, Δ25-27, G142D, V213G, G339D, S371F, S…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

July 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLM-AMA18.0 dCas9-VPR

Plasmid

#138945

Purpose

Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

Depositor

Arnold Driessen

Publication

Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

Insert

p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers

Use

CRISPR and Synthetic Biology; Ama1 autonomously r…

Tags

NLS

Promoter

p40S (AN0465) Aspergillus nidulans

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

Plasmid

#99144

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

Depositor

Robert Bradley

Publication

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBAD-TRP120

Plasmid

#71732

Purpose

inducible expression of TRP120 in E. coli

Depositor

Jere McBride

Publication

Luo et al Clin Vaccine Immunol. 2009 Jul;16(7):982

Insert

TRP120

Tags

6xHIS and Thioredoxin

Expression

Bacterial

Promoter

pBAD

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pEx-deletion-tet

Plasmid

#172214

Purpose

For allelic exchange in tetracyclin-sensitive Prevotella copri strains

Depositor

Till Strowig

Publication

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Allelic exchange in prevotella copri

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAC1804_pmax-dCasRx-RBM38

Plasmid

#118638

Purpose

Transient transfection; Expresses dCasRx-RBM38; CAGGS promoter

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCasRx-RBM38

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-HxB.S

Plasmid

#65415

Purpose

contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Publication

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

tenascin C small splice variant (TNC Human)

Expression

Bacterial

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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V-Kras

Plasmid

#27806

Depositor

Steven Vogel

Publication

Koushik et al J Biomed Opt. 2008 May-Jun;13(3):031204.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Venus kras

Expression

Mammalian

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pEN35-CDKN2A-Ex2-R

Plasmid

#110737

Purpose

Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Depositor

Robert Judson-Torres

Publication

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDKN2A gRNA (CDKN2A Human)

Use

CRISPR

Expression

Mammalian

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEN35-CDKN2A-Ex2-L

Plasmid

#110736

Purpose

Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

Depositor

Robert Judson-Torres

Publication

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CDKN2A gRNA (CDKN2A Human)

Use

CRISPR

Expression

Mammalian

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TRAIP-SFB

Plasmid

#101769

Purpose

Mammalian expression of TRAIP fusion protein

Depositor

Michael Huen

Publication

Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAIP / RNF206 (TRAIP Human)

Use

Lentiviral

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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