We narrowed to 10,582 results for: plasmids 101

1. pAAV_18xTEAD_luc2

   Plasmid

   #173003

   Purpose

   firefly luciferase reporter vector with 18x clustered TEAD binding sites

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   18xTEAD-luc2
   

   Use

   AAV and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTM_COV2_NSP5_004_SUMO

   Plasmid

   #190062

   Purpose

   Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   3CLpro (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. kiCAP-AAV-PAL1C

   Plasmid

   #196685

   Purpose

   Rep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Tags

   Expression

   Mutation

   PTQGTLR insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV_12xTEAD_luc2

   Plasmid

   #173004

   Purpose

   firefly luciferase reporter vector with 12x clustered TEAD binding sites

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   12xTEAD-luc2
   

   Use

   AAV and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX C_K nsp1 CoV2

   Plasmid

   #175516

   Purpose

   Expresses C_K for bioconjugation on nsp1

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   C_K nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX nsp1 R99A CoV2

   Plasmid

   #175515

   Purpose

   For protein expression of R99A nsp1 CoV2

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   R99A nsp1 CoV2
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available Since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

   Plasmid

   #109318

   Purpose

   Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   SaCas9 RuvC gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. kiCAP-AAV-MDV1B

    Plasmid

    #196680

    Purpose

    Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    KTVGTVY insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCHC024 (∆SH)

    Plasmid

    #226202

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for soluble hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxFUYHWI ∆hypA2B2F2

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCHC023 (∆MBH)

    Plasmid

    #226201

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for membrane bound hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCHC027 (∆pemK)

    Plasmid

    #226204

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Upstream/downstream homology arms for addiction system toxinPemK.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAAV_8xTEAD_luc2

    Plasmid

    #173005

    Purpose

    firefly luciferase reporter vector with 8x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    8xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. kiCAP-AAV-PAL1D

    Plasmid

    #196686

    Purpose

    Rep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    PTQGTFR insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. kiCAP-AAV-PAL1B

    Plasmid

    #196684

    Purpose

    Rep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    PSQGTLR insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. kiCAP-AAV-PAL1A

    Plasmid

    #196683

    Purpose

    Rep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.

    Depositor

    Pardis Sabeti

    Article

    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    PTQGTVR insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available Since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-AcrIIA1(T16A)-NLS(SV40) (KAC511)

    Plasmid

    #133797

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T16A) with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1(T16A)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    T16A

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-AcrIIA1-Leu-NLS(SV40) (KAC440)

    Plasmid

    #133799

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Leu-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Leu-AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-AcrIIA1-Eriv-NLS(SV40) (KAC442)

    Plasmid

    #133800

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized Eriv-AcrIIA1 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized Eriv-AcrIIA1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge