Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,779 results for: T7

Clear keywords
Showing: 2261 - 2280 of 10779 results

  1. pJK541

    Plasmid
    #72447
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Promoter
    T7 and n/a
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72447-plasmid-map-sequence-id-133869

    Enlarge

  2. pJK543

    Plasmid
    #72516
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    contains additional Ala2
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72516-plasmid-map-sequence-id-132549

    Enlarge

  3. pJK587

    Plasmid
    #71696
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes cysteine-138 to serine
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71696-plasmid-map-sequence-id-131733

    Enlarge

  4. pJK601

    Plasmid
    #71606
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe mutant (AaPurE1-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tyrosine-154 to phenylalanine, additional…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71606-plasmid-map-sequence-id-131683

    Enlarge

  5. 4Z31

    Plasmid
    #69958
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H2:YTC020-E10:C234061 (RC3H2 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. 4Z0O

    Plasmid
    #69954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. 4QQM

    Plasmid
    #61950
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK:JMC029-A03:C213522 (RFXANK Human)
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. 2I7C

    Plasmid
    #60124
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PY03671:MAC01H-E01:C21952
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Nov. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 4IIM

    Plasmid
    #51325
    Depositor
    Cheryl Arrowsmith
    Insert
    4IIM (ITSN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. 4IIO

    Plasmid
    #51326
    Depositor
    Cheryl Arrowsmith
    Insert
    4IIO (ITSN2 4IIO, Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pHis6-SUMO-g0s2

    Plasmid
    #179293
    Purpose
    E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do
    Insert
    G0S2 (G0S2 Human)
    Tags
    His6 + SUMO + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    full-length G0S2, residues 1-103 from accession n…
    Promoter
    T7
    Available Since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179293-plasmid-map-sequence-id-354916

    Enlarge

  17. pGEMHE-RnCENP-O-298-5NS-GFP

    Plasmid
    #205742
    Purpose
    Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcription
    Depositor
    Michael Lampson
    Article
    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208
    Insert
    CENP-O (Cenpo, Cenpo Mouse, Rat)
    Use
    In vitro transcription
    Tags
    5 glycines linker followed by GFP
    Expression
    Mammalian
    Mutation
    Chimeric rat CENP-O with mouse CENP-O C-terminal …
    Promoter
    T7
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    205742-plasmid-map-sequence-id-405893

    Enlarge

Showing: 2261 - 2280 of 10779 results