We narrowed to 5,546 results for: KRAS

1. pQCXIX_sh_HOIP/RNF31_#6

   Plasmid

   #75252

   Purpose

   retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   shRNA against human RNF31 (RNF31 )
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Promoter

   Chicken ß-actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-WT SpCas9-NLS-6xHis

   Plasmid

   #207373

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   WT SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEmerald-Caveolin-C-10

   Plasmid

   #54025

   Purpose

   Localization: Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

   Insert

   Caveolin (CAV1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pcDNA-D2s-L-Venus

   Plasmid

   #19966

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)
   

   Tags

   Flag and monomeric Venus

   Expression

   Mammalian

   Available Since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERH-ZNF281

   Plasmid

   #222923

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZNF281 (ZNF281 Human)
   

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP786_SpyCatcher-EZH2-FL-CO

   Plasmid

   #211779

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-KRAB
   

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9 TadCBEe

   Plasmid

   #193836

   Purpose

   Express TadCBEe (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDe-SpCas9D10A-2xUGI
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_CamKIIa_mKate2

   Plasmid

   #96942

   Purpose

   To label mature excitatory iPSC-derived human neurons

   Depositor

   Oleksandr Shcheglovitov, Ricardo Dolmetsch

   Article

   Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

   Insert

   far-red fluorescent protein
   

   Use

   Lentiviral

   Mutation

   E87D (please see depositor comments below)

   Promoter

   CaMKIIa

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pProEx M-Ras(G22C) CL

    Plasmid

    #224268

    Purpose

    Protein purification of M-Ras (G22C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    M-Ras (MRAS Human)
    

    Expression

    Bacterial

    Mutation

    G22C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pProEx Rit1(G47C) CL

    Plasmid

    #224267

    Purpose

    Protein purification of Rit1 (G47C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rit1 (RIT1 Human)
    

    Expression

    Bacterial

    Mutation

    G47C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pProEx Ypt1(S17C) CL

    Plasmid

    #224265

    Purpose

    Protein purification of Ypt1 (S17C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Ypt1 (YPT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    S17C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2T-cmv-SpCas9-TadDE-BlastR

    Plasmid

    #193850

    Purpose

    Express TadDE in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadDE
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHDR2_DAZL-T2A-mGreenLantern-PuroTK

    Plasmid

    #222917

    Purpose

    Homology directed repair template for knocking in mGreenLantern reporter to DAZL.

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    mGreenLantern
    

    Use

    CRISPR

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA179

    Plasmid

    #215939

    Purpose

    Fragmid fragment: (guide cassette) short sequence with no known function

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Filler
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. eGFP-pk-S65A

    Plasmid

    #115619

    Purpose

    Expresses human Parkin S65A in mammalian cells

    Depositor

    Edward Fon

    Article

    Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

    Insert

    Parkin (PRKN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S65A

    Promoter

    CMV

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AA331

    Plasmid

    #215954

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL-gRNA(anti-cGAS)-Cas9-Puro

    Plasmid

    #130908

    Purpose

    gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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