We narrowed to 31,641 results for: LIS;

1. IDE-2Strep

   Plasmid

   #210355

   Purpose

   Expresses IDE fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   IDE (IDE Human)
   

   Tags

   2Strep

   Expression

   Mammalian

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-SA-neo-CAGGS-PE2-2A-GFP

   Plasmid

   #180014

   Purpose

   Targeting vector for knocking in constitutively expressed PE2-2A-GFP into the human AAVS1 locus

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   PE2-2A-GFP
   

   Use

   CRISPR; Human genome engineering

   Tags

   GFP

   Promoter

   CAG

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCASclos

   Plasmid

   #73640

   Purpose

   Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824

   Depositor

   Sheng Yang

   Article

   Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

   Inserts

   dCas9
   sgRNA to spo0A
   

   Use

   E.coli-clostridium shuttle vector

   Mutation

   D10A, H840A

   Promoter

   Pj23119 and ptb

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN-dCas9-CDA1-UGI

   Plasmid

   #196248

   Purpose

   Plasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitor

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL4-RecApro-dCas9-CDA1-UGI-attR1
   

   Expression

   Bacterial

   Promoter

   RecA promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV3Tag8li-hDDX50-HA

   Plasmid

   #165465

   Purpose

   Human DDX50 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   DExD-box helicase 50 (DDX50 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330.Pten.b

   Plasmid

   #66588

   Purpose

   sgRNA for Pten deletion

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   Pten deletion (Pten Mouse)
   

   Expression

   Mammalian

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-mPCSK8

   Plasmid

   #122677

   Purpose

   Expresses mouse PCSK8 in mammalian cells

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
   

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-VHL W117R-pBabe-puro

    Plasmid

    #19239

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tryptophan 117 to Arginin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

12. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FMR1-E17C-3xFLAG

    Plasmid

    #157784

    Purpose

    donor plasmid for C-terminal FLAG tag fusion of human FMR1

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1-FLAG (FMR1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

    Plasmid

    #197952

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26 and pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-dCpf1-eBE-YE

    Plasmid

    #107689

    Purpose

    Expresses dLbCpf1-eBE-YE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE-YE
    

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mApple-ERM

    Plasmid

    #217377

    Purpose

    ER marker

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

    Plasmid

    #105039

    Purpose

    Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Tsc2 (Tsc2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197979

    Purpose

    T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pco-vCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    pUBQ10

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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