We narrowed to 3,144 results for: PPL;

1. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

   Plasmid

   #224488

   Purpose

   Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-ArchT-tdTomato

   Plasmid

   #28305

   Purpose

   Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV5

   Insert

   ArchT-tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. FASTHDR-tRNApromo-sgRNA-espCas91_1

   Plasmid

   #167210

   Purpose

   Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV2_CAG_oROS-HT_WPRE

   Plasmid

   #216414

   Purpose

   Encodes the genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

   Plasmid

   #229812

   Purpose

   A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-mCherry
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME Lamin B Vhh mNeonGreen-pA (JDW 1312)

   Plasmid

   #224501

   Purpose

   Gateway compatible middle entry clone containing Lamin B nanobody fused to mNeonGreen with HA Tag (For visualizing the nuclear lamina, lamin chromobody)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lamin B Vhh mNeonGreen-pA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. hCXCR4-mTQ2

   Plasmid

   #110196

   Purpose

   Encodes for human CXCR4 coding sequence tagged with the mTQ2 FP

   Depositor

   Sergi Padilla Parra

   Article

   Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

   Insert

   hCXCR4 (CXCR4 Human)
   

   Use

   Tags

   mTurquoise (mTQ2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1-dmito-oROS-HT

   Plasmid

   #216418

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   dmito-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Epac-S-H81

   Plasmid

   #170339

   Purpose

   GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

    Plasmid

    #224489

    Purpose

    Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Sun1-2xsfGFP-6xMYC-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-ArchT-GFP

    Plasmid

    #28307

    Purpose

    Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-oROS-HT-CaaX

    Plasmid

    #216417

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the intracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT-CaaX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. hCXCR4-LSSmOrange

    Plasmid

    #110197

    Purpose

    Encodes for human CXCR4 coding sequence tagged with the LSS-mOrange FP

    Depositor

    Sergi Padilla Parra

    Article

    Starling et al Nat Commun. 2023 Aug 17;14(1):4994. doi:

    Insert

    hCXCR4 (CXCR4 Human)
    

    Use

    Tags

    LSSmOrange

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-oROS-HT

    Plasmid

    #216416

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the extracellular site of cell membranes.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    pDisplay-oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)

    Plasmid

    #224500

    Purpose

    Gateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mNeonGreen-HA-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

    Plasmid

    #35509

    Purpose

    AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV5 and AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. Gfp-rt-vasa

    Plasmid

    #167322

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    rt vasa 3'UTRs (ddx4 rainbow trout)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p5E-MCS-c-Fos-b-globin (JDW 1237)

    Plasmid

    #229829

    Purpose

    A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    c-fos minimal promoter and b-globin intron
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Epac-S-H90

    Plasmid

    #170340

    Purpose

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

    Insert

    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge