We narrowed to 5,570 results for: donor

1. pTLR repair vector

   Plasmid

   #64322

   Purpose

   Vector for repair of the traffic light reporter (TLR), providing the venus coding region

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Insert

   Venus
   

   Use

   Artificial targeting vector

   Mutation

   see publication for details

   Promoter

   none

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. KHBD00188

   Plasmid

   #34340

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG1034 (bcd Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Nanog-2A-mCherry

   Plasmid

   #48680

   Purpose

   For knock-in donor vector by CRISPR/CAS system

   Depositor

   Rudolf Jaenisch

   Article

   Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

   Insert

   Nanog (Nanog Mouse)
   

   Use

   CRISPR

   Tags

   Cherry

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. INS-2A-luciferase-2A-tdT

   Plasmid

   #159348

   Purpose

   Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Insert

   Luciferase-2A-tdTomato
   

   Use

   Ins locus knockin donor vector

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ULK1-Halo HRD

   Plasmid

   #207548

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMN-009

   Plasmid

   #195413

   Purpose

   AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA
   

   Expression

   Mammalian

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCPP5154

   Plasmid

   #128704

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   avrPto
   

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCPP5616

   Plasmid

   #128727

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   avrPtoB
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCPP5534

   Plasmid

   #128723

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopX1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1-GT_CAG_3xTurboGFP-NLS

    Plasmid

    #229777

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    3x TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Bxb1-GA_CAG-TurboGFP

    Plasmid

    #229781

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TurboGFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLP-pR-himar-apraR

    Plasmid

    #199996

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19_dTAG-KANSL3-targeting

    Plasmid

    #170755

    Purpose

    Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

    Depositor

    Kristian Helin

    Article

    Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

    Insert

    BSD-P2A-2xHA-dTAG
    

    Use

    Mouse Targeting

    Tags

    BSD-P2A-2xHA-dTAG

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLP-pTac-Tn5-chlorR

    Plasmid

    #199994

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. N-terminal Halo-XLF

    Plasmid

    #207546

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCPP5238

    Plasmid

    #128711

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopG1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCPP5537

    Plasmid

    #128730

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    HopAO1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ145

    Plasmid

    #98405

    Purpose

    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge