We narrowed to 5,258 results for: pcdna3.1

1. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

   Plasmid

   #127326

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

   Plasmid

   #50866

   Purpose

   Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C I730C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

   Plasmid

   #50870

   Purpose

   Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   N680C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 Flag YFP hNKCC1 P676C A734C (NT810)

   Plasmid

   #50867

   Purpose

   Expresses human NKCC1 P676C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 Flag YFP hNKCC1 A675C A734C (NT841)

   Plasmid

   #50864

   Purpose

   Expresses human NKCC1 A675C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   A675C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 Flag YFP hNKCC1 S679C I730C (NT838)

   Plasmid

   #50869

   Purpose

   Expresses human NKCC1 S679C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   S679C I730C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 Flag YFP hNKCC1 I674C I730C (NT835)

   Plasmid

   #50863

   Purpose

   Expresses human NKCC1 I674C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   I674C I730C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)

   Plasmid

   #50868

   Purpose

   Expresses human NKCC1 I677C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   I677C A734C in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

   Plasmid

   #49080

   Purpose

   Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Use

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   C791S, C820S, C1052S in hNKCC1 in NT17

   Promoter

   CMV

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

    Plasmid

    #49070

    Purpose

    Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    ECL4 deletion of amino acids 562-582 in hNKCC1 in…

    Promoter

    CMV

    Available since

    Nov. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)

    Plasmid

    #49073

    Purpose

    Expresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C723S,C724V in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid

    #49062

    Purpose

    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    N-terminal deletion of amino acids 13-222 in hNKC…

    Promoter

    CMV

    Available since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

    Plasmid

    #111460

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346A, C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

    Plasmid

    #111461

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346D, C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

    Plasmid

    #138610

    Purpose

    Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    Hyperactive AID
    T7 RNA Polymerase
    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST

    Plasmid

    #127332

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

    Plasmid

    #63565

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

    Plasmid

    #47387

    Purpose

    allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    glutamate-gated chloride Channels
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid

    #140893

    Purpose

    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

    Depositor

    Yasushi Okamura

    Article

    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

    Insert

    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

    Promoter

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735

    Plasmid

    #60633

    Purpose

    ZKSCAN1 circular RNA expression plasmid containing minimal sufficient introns

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge