We narrowed to 165,918 results for: addgene

1. pGH224_sgRNA_2xMS2_Puro

   Plasmid

   #85413

   Purpose

   hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. E[beta]C

   Plasmid

   #24312

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)
   

   Use

   Lentiviral

   Mutation

   Sites phosphorylated by GSK-3beta have been mutat…

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. HLA_A0101

   Plasmid

   #165009

   Purpose

   Expression of HLA-A*01:01 cDNA

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   HLA-A*01:01 (HLA-A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACUH

   Plasmid

   #58374

   Purpose

   UAS vector to express gene of interest in Drosophila

   Depositor

   Chun Han, Yuh-Nung Jan

   Article

   Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Expression

   Insect

   Promoter

   15xQUAS-TATA

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBI-MCS-EYFP

   Plasmid

   #58855

   Purpose

   The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   enhanced yellow fluorescent protein
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTlpA_mCherry

   Plasmid

   #229146

   Purpose

   Promoter strength evaluation of PtlpA

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry
   

   Expression

   Bacterial

   Mutation

   No

   Promoter

   PtlpA

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCrhaB2plus

   Plasmid

   #164226

   Purpose

   Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.

   Depositor

   Silvia Cardona

   Article

   Hogan et al Appl Environ Microbiol. 2021 Jun 30:AEM0

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Rhamnose-inducible

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459_gRNA pAS dual_hspCas9

   Plasmid

   #193312

   Purpose

   Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS dual sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPE4max-Blasticidin

    Plasmid

    #194905

    Purpose

    Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

    Depositor

    George Church

    Article

    Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

    Insert

    BSD
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GalToxBFP

    Plasmid

    #68073

    Purpose

    mammalian expression of GC localized oxBFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    GalT signal anchor transmembrane domain
    

    Tags

    moxBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459_gRNA-ROSA26_hspCas9

    Plasmid

    #193310

    Purpose

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    not applicable

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-GfaABC1D-GRAB_Ado1.0

    Plasmid

    #153288

    Purpose

    Express the adenosine sensor GRAB_Ado1.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

    Insert

    Adenosine sensor GRAB_Ado1.0
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIG-Aalpha WT

    Plasmid

    #10884

    Depositor

    William Hahn

    Article

    Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

    Insert

    PP2A Aalpha (PPP2R1A Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVPT-tTR-KRAB

    Plasmid

    #11642

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_CMV::rtTA3-bGHpA

    Plasmid

    #62445

    Purpose

    MXS_chaining vector with CMV::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with CMV promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVET-tTR-KRAB

    Plasmid

    #11644

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hEF-1alpha, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. 7MEQ

    Plasmid

    #176412

    Purpose

    Baculovirus expression for structure determination. May not contain entire coding region of gene.

    Depositor

    Cheryl Arrowsmith

    Insert

    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    

    Use

    Baculovirus expresssion

    Tags

    8 x His Tag and Honeybee melittin signal

    Mutation

    etmprss2_S251D/R252D/Q253D/S254D/R255K

    Promoter

    Polyhedrin

    Available Since

    Oct. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pNup133-Ypet

    Plasmid

    #98283

    Purpose

    mammalian expression of Nup133-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup133 (NUP133 Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE puro PPAR gamma 2

    Plasmid

    #8859

    Depositor

    Bruce Spiegelman

    Article

    Tontonoz et al Cell. 1994 Dec 30. 79(7):1147-56.

    Insert

    PPAR gamma (Pparg Mouse)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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