We narrowed to 162,449 results for: addgene

1. pGKO2-Gateway

   Plasmid

   #63618

   Purpose

   a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.

   Depositor

   Seogchan Kang

   Article

   Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

   Type

   Empty backbone

   Use

   Fungal expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. rtTA and TagBFP cassettes

   Plasmid

   #62452

   Purpose

   MXS_chaining vector with 2 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::NLS-TagBFPx3-bGHpA
   CMV::rtTA3-bGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide-BC-EF1a-Puro

   Plasmid

   #127170

   Purpose

   PCR template for EF1a-puro resistance cassette

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPRv2-Stuffer-HepmTurquoise2

   Plasmid

   #192826

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mTurquoise2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

   Plasmid

   #75295

   Purpose

   An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

   Depositor

   Brandon Harvey

   Insert

   eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human SYN1

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MXS_CMV::HygroR-bGHpA

   Plasmid

   #62435

   Purpose

   MXS_chaining vector with CMV::HygroR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against hygromycin B with CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MXS_PGK::rtTA3-bGHpA

   Plasmid

   #62446

   Purpose

   MXS_chaining vector with PGK::rtTA3-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the reverse tetracycline transactivator with PGK promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MXS_PGK::HygroR-bGHpA

   Plasmid

   #62436

   Purpose

   MXS_chaining vector with PGK::HygroR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against hygromycin B with PGK Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. lag-2 promoter:GFP (pJK590)

    Plasmid

    #26814

    Depositor

    Judith Kimble

    Article

    Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

    Insert

    lag-2 promoter
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. TAL2472

    Plasmid

    #36853

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    TP53-TALEN_Right (Tp53 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TAL2348

    Plasmid

    #36768

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    TP53-TALEN_Left (Tp53 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCM-HA-Gas1

    Plasmid

    #38313

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    Gas1 (GAS1 Budding Yeast)
    

    Use

    Tags

    2xHA

    Expression

    Mutation

    contains promoter region

    Promoter

    Endogenous

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. MXS_PGK::CreERT2-bGHpA

    Plasmid

    #62444

    Purpose

    MXS_chaining vector with PGK::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. FUap97GW (C4)

    Plasmid

    #74020

    Purpose

    lentiviral expression of SAP97alpha-EGFP fusion

    Depositor

    Robert Malenka, Oliver Schluter, Weifeng Xu

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    SAP97 alpha
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiGuide-BC-CMV-Puro

    Plasmid

    #127169

    Purpose

    PCR template for CMV-puro resistance cassette

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. EmptyTranscriptionalReporter

    Plasmid

    #62453

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMVmin::NLS-Citrine-bGHpA
    CMV::NLS-Cherry-bGHpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR_eGFP_369

    Plasmid

    #111823

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 349-369
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_CMV::CreERT2-bGHpA

    Plasmid

    #62443

    Purpose

    MXS_chaining vector with CMV::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pN-PURO-PTP

    Plasmid

    #51711

    Purpose

    contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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