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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 2321 - 2340 of 173477 results

  3. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #219653
    Purpose
    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus
    Depositor
    Thomas Oertner
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)
    Expression
    Mammalian
    Promoter
    nEf Addgene # 149296
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    219653-plasmid-map-sequence-id-434847

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  4. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

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  15. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

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  18. 6DXH (USP05)

    Plasmid
    #119968
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    USP05 (USP5 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 171-290
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119968-plasmid-map-sequence-id-232692

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  19. 6DUB (LOC149281)

    Plasmid
    #119967
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    LOC149281 (METTL11B Human)
    Tags
    6xHIS, thrombin,SUMO and TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acids 58-278
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119967-plasmid-map-sequence-id-232691

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  20. FOXN1 (FOXN1A-c017)

    Plasmid
    #110249
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FOXN1 (FOXN1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110249-plasmid-map-sequence-id-212285

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Showing: 2321 - 2340 of 173477 results