We narrowed to 5,646 results for: donor

Showing: 2321 - 2340 of 5646 results

Results per page:

Sort results by:

pSLHDR03_mCherry_tCD19_unique guide

Plasmid

#124062

Purpose

Construction of 1st donor for scarless genome editing with unique guide

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

mCherry
tCD19

Use

Mouse Targeting; Human targeting

Promoter

Human UbC modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT-CAG-mStayGold

Plasmid

#241387

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CAG promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC_mClover3_BSDminus_F2

Plasmid

#184167

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC_mClover3_BSDminus_F1

Plasmid

#184166

Purpose

Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mClover3
bsd

Use

CRISPR

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Nanog-2A-mCherry

Plasmid

#48680

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Nanog (Nanog Mouse)

Use

CRISPR

Tags

Cherry

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-PtH2B

Plasmid

#133390

Purpose

Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

Depositor

Blair Madison

Article

Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

Type

Empty backbone

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-013

Plasmid

#195417

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF5

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF5 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5052

Plasmid

#128729

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAM1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX552-mScn2a-3xV5-EF1-smFP-flag

Plasmid

#182561

Purpose

For mouse Nav1.2 knockin with 3xV5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn2a gRNA and 3xV5 donor DNA
smFP-flag

Use

AAV and CRISPR

Promoter

EF1 and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

Plasmid

#154834

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Inserts

ETN
ETN

Use

Cre/Lox

Expression

Mammalian

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
INS-2A-luciferase-2A-tdT

Plasmid

#159348

Purpose

Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

Luciferase-2A-tdTomato

Use

Ins locus knockin donor vector

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEED-LoxP-sfGFP:ALFA

Plasmid

#219660

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette (LoxP)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-009

Plasmid

#195413

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5534

Plasmid

#128723

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopX1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5154

Plasmid

#128704

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

avrPto

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ULK1-Halo HRD

Plasmid

#207548

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5616

Plasmid

#128727

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

avrPtoB

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC1821_pCR8-SMN2-DN-RG6-SA

Plasmid

#118655

Purpose

Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

SMN2-DN-RG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT_CAG_3xTurboGFP-NLS

Plasmid

#229777

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

3x TurboGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCPP5238

Plasmid

#128711

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopG1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge