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pMYT013

Plasmid

#180666

Purpose

Encodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet5

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P*-R

Plasmid

#189850

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P*-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P* (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFH114

Plasmid

#128408

Purpose

Level1 ZmUBIp::BAR:NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level1 ZmUBIp::BAR:NOSt (Position 1)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJA252

Plasmid

#21512

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5 (w/o ATG)

Use

Multisite gateway

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P**-R

Plasmid

#189851

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P**-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P** (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-cat-P-R

Plasmid

#189853

Purpose

Cre-inducible activation of cat for chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-cat

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GNb-RNb

Plasmid

#128787

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-RNb

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMLS134

Plasmid

#73714

Purpose

SapTrap destination vector for building sgRNA expression vectors.

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pStrep-1xEGFP-N1

Plasmid

#122488

Purpose

Expression of Strep-tagged 1xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 1xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMYT018

Plasmid

#180671

Purpose

Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

rtTA

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT016

Plasmid

#180669

Purpose

Encodes Z3EV as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

Z3EV

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT015

Plasmid

#180668

Purpose

Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet7

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT001

Plasmid

#180654

Purpose

Encodes pZ3 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pZ3

Use

Synthetic Biology

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT095

Plasmid

#198866

Purpose

CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23106-B0034m-E0040m-B0015)

Plasmid

#197671

Purpose

Expresses GFP under the control of J23106 and B0034m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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RNb-FKBP

Plasmid

#128779

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

RNb-FKBP

Expression

Mammalian

Available Since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GNb-FKBP

Plasmid

#128771

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-FKBP

Expression

Mammalian

Available Since

Feb. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pStrep-2xEGFP-N1

Plasmid

#122489

Purpose

Expression of Strep-tagged 2xEGFP

Depositor

Georg Mayr

Article

Zweifel et al Anal Biochem. 2019 Sep 12:113430. doi: 1

Insert

Strep-tagged 2xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFH22

Plasmid

#125808

Purpose

Level0 xCas9 3.7

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

xCas9 3.7

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-B0034m-E0040m-B0015)

Plasmid

#197659

Purpose

Expresses GFP under the control of J23100 and B0034m

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge