We narrowed to 6,093 results for: pcDNA 3.1

1. pcDNA3.1(+) Laccase2 MCS-ZKSCAN1 548-1417 (No intron)

   Plasmid

   #69898

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (stw, ZKSCAN1 Human)
   

   Expression

   Mammalian

   Mutation

   F169L in ZKSCAN1

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

   Plasmid

   #171375

   Purpose

   For the mammalian expression of rat Gαi3 internally tagged with YFP

   Depositor

   Mikel Garcia-Marcos

   Article

   Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

   Insert

   Gai3-YFP
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

   Plasmid

   #58275

   Purpose

   mammalian expression of human RdgBbeta splice variant 1

   Depositor

   Shamshad Cockcroft

   Article

   Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

   Insert

   RdgBbeta (PITPNC1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

   Plasmid

   #168099

   Purpose

   HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nUbc-OGA(544-706)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)

   Plasmid

   #51369

   Depositor

   Narry Kim

   Article

   Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

   Insert

   Dicer (DICER1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   3' pocket mutant (Y926F, R927A)

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-sfVenus-iGluSnFR

   Plasmid

   #187097

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Pre-SPHERE-sfVenus-iGluSnFR
   

   Tags

   TagBFP-P2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR

   Plasmid

   #187098

   Purpose

   Glutamate sensor for localization to membrane contatct

   Depositor

   Kotaro Oka

   Article

   Shindo et al bioRxiv 2022

   Insert

   Post-SPHERE-sfVenus-iGluSnFR
   

   Tags

   iRFP670-P2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

   Plasmid

   #49061

   Purpose

   Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5065 pcDNA3.1/V5-His-TOPO hBrd4 (888-1362)

   Plasmid

   #14444

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 (888-1362) (BRD4 Human)
   

   Expression

   Mammalian

   Mutation

   amino acids 888-1362

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)

    Plasmid

    #69904

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Expression

    Mammalian

    Mutation

    F169L in ZKSCAN1

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. (369) pcDNA3.1(+)-HA-nLaG6-(EAAAK)4-OGA(544-706)

    Plasmid

    #168190

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGA with residues 544-706. For use with myc-OGA(1-400) split-OGA system.

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGA(544-706)
    

    Tags

    HA-Tag

    Expression

    Mammalian

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

    Plasmid

    #73210

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Synthetic, Human)
    

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid

    #49069

    Purpose

    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C563S,C568S,C577S,C582S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP G protein Gamma 3(A73L,L74I,L75S) in pcDNA3.1

    Plasmid

    #42190

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

    Insert

    G protein Gamma 3(A73L,L74I,L75S) (Gng3 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A73L,L74I,L75S

    Promoter

    CMV

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1-iRFP-P2A-Post-SPHERE-sfmTurquoise2-iGluSnFR

    Plasmid

    #187096

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Post-SPHERE-sfmTurquoise2-iGluSnFR
    

    Tags

    iRFP670-P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225588

    Purpose

    Mammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-F8.2(S54L+T127A)-T2A-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

    Plasmid

    #227620

    Purpose

    Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257A,R347A

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-S1332A/S1346A

    Plasmid

    #215902

    Purpose

    Expression of TNRC6A-CIM1 domain with S1332A/S1346A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528, S1332A/S1346A

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3.1(+)-hSyn-HA-mTREK-1(S131C/A286F)-IRES-GFP

    Plasmid

    #225490

    Purpose

    Neuronal expression IRES-containing bicistronic vector for generating mTREK-1 (S131C/A286F) + EGFP under the hSyn promoter.

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    Neuronal expression

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

    Plasmid

    #217478

    Purpose

    Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

    Depositor

    Peng Zou

    Article

    Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

    Insert

    Nier1s
    

    Tags

    3xNLS, P2A-EGFP-3xNLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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