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Showing: 5851 - 5900 of 6949 results

  9. pEcNucC

    Plasmid
    #214050
    Purpose
    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    EcNucC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    lacUV5 promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214050-plasmid-map-sequence-id-428997

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  34. mitoTALECD for_atp1_1397NC

    Plasmid
    #186204
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Arabidopsis RPS5A
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186204-plasmid-map-sequence-id-368339

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  35. mitoTALECD for_-20G_1333CN

    Plasmid
    #186200
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186200-plasmid-map-sequence-id-368306

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  36. mitoTALECD for_ -13G_1397CN

    Plasmid
    #186199
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186199-plasmid-map-sequence-id-368290

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  37. mitoTALECD for_ -6G_1333CN

    Plasmid
    #186198
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186198-plasmid-map-sequence-id-368288

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  45. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

    Plasmid
    #128698
    Purpose
    simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells
    Depositor
    Igor Weber
    Article
    Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11
    Inserts
    iqgC (full length)
    Raf1 kinase (aa 50-132)
    Use
    Dictyostelium discoideum expression vector
    Tags
    YFP and mRFP
    Expression
    Mutation
    none
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128698-plasmid-map-sequence-id-246776

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  46. pABD823

    Plasmid
    #67911
    Purpose
    Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    Cold temperature germinating 10 (CTG10)
    Use
    Tags
    GAL4 DNA binding domain (148 amino acids)
    Expression
    Yeast
    Mutation
    Promoter
    pADH1 (alcohol dehydrogenase 1 promoter)
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  47. pABD933

    Plasmid
    #67917
    Purpose
    Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    CTG10
    Use
    Gateway cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  50. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid
    #118269
    Purpose
    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    Use
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…
    Promoter
    T7 Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118269-plasmid-map-sequence-id-228542

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Showing: 5851 - 5900 of 6949 results