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pGSTag Flag Jnk3a1(2nd methione)

Plasmid

#15746

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1-2nd M (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pGEX4T-Mst1 SARAH

Plasmid

#84297

Purpose

Expresses Mst1 SARAH domain for interaction studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst1 (STK4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Meis2

Plasmid

#74349

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 2 (Meis2 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer21 Flag-Sesn1b

Plasmid

#61865

Purpose

Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pGSTag Flag Jnk3a2(2nd methione)

Plasmid

#15747

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2(2nd methione) (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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HOXD13 (chicken) HIS-tag pET

Plasmid

#8555

Depositor

Corey Largman

Article

Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

Insert

HOXD13 (HOXD13 )

Tags

His and T7

Expression

Bacterial

Mutation

contains 10 nt 5' to ATG start; makes prote…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pISH-Meis1

Plasmid

#74348

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Meis1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Meis homeobox 1 (Meis1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
M71::Myc6-IRES-taulLacZ LNL TV

Plasmid

#15619

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::Myc6-IRES-taulLacZ LNL TV (Myc Mouse)

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE58

Plasmid

#247035

Purpose

IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF1

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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PX459-sgRNA047_Podxl

Plasmid

#232932

Purpose

Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Podxl S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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MPS1 Human

Plasmid

#232149

Purpose

MPS1 from Human codon optimized for S. cerevisiae in pUCIDT

Depositor

Mark Winey

Article

Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

Insert

MPS1 (TTK Human)

Expression

Yeast

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-G-G mm

Plasmid

#221728

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with G-G mismatch at position 6 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-G-G mm

Use

Fish expression

Mutation

G-G mismatch of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-Zeo-miR-430-10-11 mm

Plasmid

#221727

Purpose

Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

BleoR-miR-430-10-11 mm

Use

Fish expression

Mutation

Mismatch at positions 10 and 11 of miR-430 site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-I304N

Plasmid

#225461

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (I304N)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

I304N

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKH160_FLAG-APEX2-YIPF4 LIR mut

Plasmid

#214979

Purpose

Flag-APEX2 tagged LIR mutant YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2_YIPF4_mut (YIPF4 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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