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Plasmid#198287PurposeProduces PVCs retargeted with anti-EGFR DARPin E01DepositorInsertPVCpnf_pvc13-E01DARPin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
UseTagsHis-TagExpressionBacterialMutationPromoterTrcAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12CTermTAG
Plasmid#125267PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertCTermTAG
UseTagsHis-TagExpressionMutationPromoterTrcAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Asp73TAG
Plasmid#125266PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Asp73TAG
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-7D12Gln13TAG
Plasmid#125264PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsertGln13TAG 7D12
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459-dErbB1
Plasmid#197354PurposeA knock-out vector for dog ErbB1.DepositorInsertA gRNA targeting the dog EGFR gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKUNOC-ErbB1-mScarlet
Plasmid#197360PurposeEncoding human ErbB1 tagged with mScarlet.DepositorInsertERBB1 (EGFR Human)
UseTagsmScarletExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her2-dL5-Her2
Plasmid#85624PurposeBacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Synthetic, Human, Schistosoma japonicum)
UseTags10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
FLT1-KDR
Plasmid#86034Purposechimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2DepositorUseTagsHA and t-GFPExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFLT1 delta CTD
Plasmid#86036Purposehuman VEGFR1 with deletion of the entire intracellular domainDepositorInserthuman Flt1 delta CTD (FLT1 Human)
UseTagsFLAG and HAExpressionMammalianMutationdeta CTDPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 Y1333F
Plasmid#86037Purposehuman VEGFR1 with Y to F mutation at position 1333DepositorInserthuman Flt1 Y1333F (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationY1333F; HA tag is inserted downstream of signal …PromoterCMVAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ubiquiton Human Kit
Plasmid Kit#1000000244PurposeThe Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.DepositorAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-m-Flt4
Plasmid#119232PurposePlasmid encodes full-length murine VEGFR-3 gene cloned in mammalian expression vector.DepositorInsertVegfr3 (Flt4 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-hR3-EGFP
Plasmid#119231PurposePlasmid encodes full-length human VEGFR-3 gene fused in-frame with EGFP cloned in lentiviral vector.DepositorInsertVEGFR3-EGFP (FLT4 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterP hUbCAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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Tol2-kdrl:mCherry-caax
Plasmid#194287PurposeExpression plasmid for zebrafish or Danionella cerebrumDepositorInsertkdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
UseZebrafish or danionella cerebrum expressionTagsmCherryCAAX from the Tol2kit (Kwan et al., 2007)ExpressionMutationPromoterkdrl(flk1)Available sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLXSN-FLK1 TM
Plasmid#65249PurposeExpression of dominant negative FLK1 in mammalian cellsDepositorInsertFLK1 TM (Kdr Mouse)
UseRetroviralTagsExpressionMammalianMutationdeleted AA 786-1346PromoterLTRAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLT1C-c201
Plasmid#205108PurposeFLT1 protein expressionDepositorInsertFLT1 (FLT1 Human)
UseTagsAviTag-6His and PTPRSspExpressionMammalianMutationWTPromoterAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV2_HLF-ZE
Plasmid#175034PurposeHLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codonDepositorInsertHLF ires ZsGreen P2A tEGFR
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 V767A
Plasmid#86038Purposehuman VEGFR1 with V to A mutation at position 767DepositorInserthuman Flt1 V767A (FLT1 Human)
UseTagsFLAG, HA (please see depositor comments below), a…ExpressionMammalianMutationV767APromoterCMVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFLT1 delta 754-763
Plasmid#86035Purposehuman VEGFR1 with a 10 amino acid deletion in EC close to TM domainDepositorInserthuman Flt1 delta754-763 aa (FLT1 Human)
UseTagsFLAG, HA, and MycExpressionMammalianMutationdelta 754-763PromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
zw3 (shaggy) pbOVI
Plasmid#38749DepositorInsertzw3 (sgg Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
myc-tagged NRP1 (human)
Plasmid#213068PurposeExpress myc-NRP1 in mammalian cellsDepositorInsertneuropilin 1 (NRP1 Human)
UseTagsMycExpressionMammalianMutationN-terminal myc tag was introduced by a three-step…PromoterT7Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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PECAM-eGFP
Plasmid#14688DepositorInsertPECAM promoter/enhancer (Pecam1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
peGFP-N1-SHB
Plasmid#139605PurposeExpresses eGFP-SHB fusion protein in mammalian cellsDepositorInsertSHB (SHB Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only