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Plasmid#225190PurposeUsed for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 siteDepositorInsertSpyCatcher003:mNeonGreen
UseTagsSpyCatcherExpressionBacterialMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK110 [Term_cat_PartType10]
Plasmid#229347PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Term_cat_PartType10DepositorInsertcat Terminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK097 [Term_cat_PartType7]
Plasmid#229334PurposeXanthoMoClo Golden Gate Part Plasmid for cloning, contains Term_cat_PartType7DepositorInsertcat Terminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
Plasmid#203964PurposeDox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)DepositorInsertSOX9-noHMG (Sox9 Mouse)
UseLentiviralTagsmycExpressionMutationthe HMG domain is truncatedPromoterTRE3GAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninV-
Plasmid#229696PurposeTransient expression of GFP-alpha-cateninV- in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationL344PPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-P-Domain
Plasmid#201193PurposeExpresses furin P-domain genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-P-Domain
UseTagsHisTag and SpyCatcher001ExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-acatenin
Plasmid#186458PurposeExpresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage siteDepositorInsertCTNNA1 (CTNNA1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P--Dr-aCateninFL
Plasmid#191740PurposeExpresses FL D rerio catenin in bacteriaDepositorInsertCTNNA1 (ctnna1 Zebrafish)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AP-SpyCatcher
Plasmid#223636PurposeProtein purification of SpyCatcher003 (S49C) with 10xHis and AP fragment of split APEX2 tags from E. coliDepositorInsertSpyCatcher003 S49C
UseTagsHis10, AP fragment of split APEX2 tagExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAT.482
Plasmid#203952PurposeSpectinomycin-based RSF1010 acceptor Level TDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_158 [TcatP]
Plasmid#210680PurposeLevel 0 partDepositorInsertTcatP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_157 [catP]
Plasmid#210679PurposeLevel 0 partDepositorInsertcatP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL0_156 [PcatP]
Plasmid#210678PurposeLevel 0 partDepositorInsertPcatP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP786_SpyCatcher-EZH2-FL-CO
Plasmid#211779PurposeSpyTag counterpart binding domain, SpyCatcher, fused to polycomb repressive complex subunit EZH2, with GFP selectionDepositorInsertaGCN4-KRAB
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP781_SnoopCatcher-KRAB-CO
Plasmid#211774PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selectionDepositorInsertTraptavidin-KRAB
UseTags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect KRAB e…PromoterpEF1a and pSV40Available sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dox-SOScat
Plasmid#207329PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)DepositorInsertson of sevenless homolog 2 variant, catalytic domain
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 593 to 1078PromoterTet Response ElementAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-DIIG
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_53 [TcatA]
Plasmid#198966PurposeLevel 0 partDepositorInsertTcatA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only