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Plasmid#221069PurposeExpression of poly H35R SUMO1 protein under T7 promoterDepositorInsertFive repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationH35RPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
H35R H43R SUMO1
Plasmid#221063PurposeExpression of H35R H43R SUMO1 protein under T7 promoterDepositorInsertH35R H43R double mutation on SUMO 1 (SUMO1 Human)
UseTagsHisExpressionBacterialMutationH35R, H43RPromoterT7Available sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)
Plasmid#231377PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH56A, K98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)
Plasmid#231378PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97A, K98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)
Plasmid#231380PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97E, K98EPromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)
Plasmid#231383PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR710A, H712APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-SeKaiA
Plasmid#229218PurposeFor expression of S. elongatus KaiA proteinDepositorInsertkaiA (kaiA S. elongatus)
UseTagsFLAG and SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
SUMO-LPQTG-pET
Plasmid#221851PurposeExpresses SUMO-2 with sortase motif and His tagDepositorInsertSUMO-2 (SUMO2 Human)
UseTagsExpressionBacterialMutationGln88Leu, Gln89ProPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Ideonella-bGSDM-WELQ
Plasmid#222098PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.DepositorInsertIdeonella-bGSDM-WELQ ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM-thrombin
Plasmid#222099PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.DepositorInsertVitiosangium-bGSDM-thrombin ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Nostoc-bGSDM-WELQ
Plasmid#222100PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.DepositorInsertNostoc-bGSDM-WELQ ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGS Q16C
Plasmid#217674PurposeFor expression of SUMO tagged human gamma S crystallin with Q16C mutation to act as a biological control for ligation experimentDepositorInsertCRYGS (CRYGS Human)
UseTags6XHis and SUMOExpressionBacterialMutationGlutamine 16 changed to CysteinePromoterT7Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 5W
Plasmid#213623PurposeBacterial expression of SUMO-tagged rat syt1 5WDepositorInsertsyt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt1 s7+
Plasmid#213624PurposeBacterial expression of SUMO-tagged rat syt1 s7+DepositorInsertsyt1 s7+ (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationL171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…PromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt1 5W
Plasmid#213629PurposeBacterial expression of SUMO-tagged rat FL-syt1 5WDepositorInsertFL-syt1 5W (Syt1 Rat)
UseTagsSUMOExpressionBacterialMutationM173W, F231W, F234W, C277A, V304W, Y364W, I367CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-FL-syt7
Plasmid#213630PurposeBacterial expression of SUMO-tagged mouse FL-syt7DepositorInsertFL-syt7 (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 43-684
Plasmid#205056PurposeTWNK protein expressionDepositorInsertTWNK (TWNK Human)
UseTagsExpressionBacterialMutationLacks amino acid from 1-42PromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80 JuxtaK-421)
Plasmid#209008PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutationsDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…PromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(143-421)
Plasmid#209007PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 143-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 43-372
Plasmid#205054PurposeTWNK protein expressionDepositorInsertTWNK (TWNK Human)
UseTagsExpressionBacterialMutationLacks Mitochondrial localization signal and C ter…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only