We narrowed to 1,010 results for: biotin
-
Plasmid#90221PurposeComplements mutations in Aspergillus nidulans gene biA (AN6644) (mutants require biotin)DepositorInsertATET_07102
UseAspergillusPromoterpLacAvailable SinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRB-pBirA-HA
Plasmid#83415Purposeproximity-directed biotin labeling on the FRB domain of mTOR in mammalian cellDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BirA-HA3-HIS3MX6
Plasmid#227975PurposeTo insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertBirA
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-BioID2-HA3-HIS3MX6
Plasmid#227977PurposeTo insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertBioID3
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip
Plasmid#178054Purposemyc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd:CMV-rtTA-IRES-birA
Plasmid#31375DepositorInsertrtTA-IRES-BirA
UseAdenoviralExpressionMammalianAvailable SinceDec. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
TGFB1-bio-His
Plasmid#52185PurposeExpresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTGFB1 (TGFB1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
FURIN-bio-His
Plasmid#51755PurposeExpresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertFURIN (FURIN Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
PDIA3-bio-His
Plasmid#52070PurposeExpresses full-length Protein disulfide-isomerase A3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDIA3 (PDIA3 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntr_Myc-BirA-G3-ER
Plasmid#173814PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseGateway shuttle vectorTagsMycAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
THBS1-bio-His
Plasmid#53417PurposeExpresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTHBS1 (THBS1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD4d3+4-bio
Plasmid#32402DepositorTypeEmpty backboneTagsbiotinylation peptide and ratCd4d3+4ExpressionMammalianPromoterCMV promoterAvailable SinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1
Plasmid#86885PurposeC-terminal part of the split-BioIDDepositorInsertBirA* (AA141-321)- HA- NIPP1]
TagsHA tagExpressionMammalianMutationBirA Amino Acids 141-321Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
LRP8-bio-His
Plasmid#51879PurposeExpresses full-length Low-density lipoprotein receptor-related protein 8 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertLRP8 (LRP8 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)
Plasmid#86886PurposeN-terminal part of the split-BioIDDepositorInsertPP1g - FLAG - BirA (AA2-140)
TagsFLAG tagExpressionMammalianMutationBirA Amino Acids 2-140Available SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD47-bio-His
Plasmid#53150PurposeExpresses full-length CD47 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD47 (CD47 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
C1QBP-bio-His
Plasmid#53330PurposeExpresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertC1QBP (C1QBP Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only