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Plasmid#90420PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NGDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_fcpA_NN
Plasmid#90419PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NNDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_fcpA_NI
Plasmid#90418PurposeTALEN Backbone for Phaeodactylum tricornutum_ fcpA promotor_NIDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM9_NR_NI
Plasmid#90422PurposeTALEN Backbone for Phaeodactylum tricornutum_ NR promotor_NIDepositorInsertTALEN Backbone
UsePhaeodactylum tricornutumTagsExpressionMutationPromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMALp2x-SCO5461
Plasmid#42507DepositorInsertguanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
UseTagsmaltose-binding proteinExpressionBacterialMutationPromotertacAvailable sinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTE1059
Plasmid#186628PurposeExpression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.DepositorInsertsscbA
scbB
scbC
UseSynthetic BiologyTags6xHis and 6xHis-thrombinExpressionBacterialMutationPromoterSynthetic promoter B10, Synthetic promoter B19, a…Available sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMALp2x-SCO5461(43-204)
Plasmid#42509DepositorInsertguanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
UseTagsmaltose-binding proteinExpressionBacterialMutationdeleted amino acids 1-42 (nucleotide 1-126)PromotertacAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMALp2x-SCO5461(E164D)(43-204)
Plasmid#42508DepositorInsertguanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
UseTagsmaltose-binding proteinExpressionBacterialMutationDeleted amino acids 1-42 (nucleotide 1-126); chan…PromotertacAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMAL-CellTag-multi-v1 barcode library
Pooled Library#206045PurposeThe CellTag multi library of barcodes can be used to clonally label cells with DNA barcodes. These barcodes can be profiled directly with single-cell RNA and ATAC sequencing, to allow clonal trackingDepositorUseLentiviralAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
S. rosetta Col+ cDNA amplified library
Pooled Library#62844Purposeamplified library of Salpingoeca rosetta from a colony rich culture which included the colony inducing bacteria Algoriphagus machipongonensisDepositorExpressionBacterialSpeciesSalpingoeca rosettaAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA:NbVPE1a/b
Plasmid#223218PurposeExpresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoterDepositorInsertNbVPE1a/b
UseCRISPR and Synthetic BiologyTagsNoneExpressionPlantMutationPromoterArabidopsis U6 promoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human SLC overexpression library
Pooled Library#213694PurposeInducible barcoded library with 467 codon-optimized cDNAs with UMIs to test the impact of SLC transporters on biological processes.DepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C2
Plasmid#141247PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-TAR C1
Plasmid#141246PurposeTransformation Associated Recombination (TAR) cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C1
Plasmid#141248PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorInsertReduced Mitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…PromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0033
Plasmid#185624PurposeLevel 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCGDepositorInsertNbU6-2
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0032
Plasmid#185623PurposeLevel 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCGDepositorInsertNbU6-1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA:NbCysP6
Plasmid#223217PurposeExpresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoterDepositorInsertNbCysP6 sgRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionPlantMutationPromoterArabidopsis U6 promoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPtGE34
Plasmid#107932PurposeExpresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
UseCRISPR and Synthetic Biology; Episomal vector for…TagsExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPtGE35
Plasmid#107999PurposeExpresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugationDepositorInsertsOriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain
UseCRISPR and Synthetic Biology; Episomal vector for…TagsCas9ExpressionMutationPromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only