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Plasmid#194341PurposepUC mini-Tn7 vector expressing LseCas13aDepositorInsertCas13a(Lse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-CreERT2
Plasmid#149437PurposeROSA26 targetting vector with tamoxifen-inducible Cre recombinaseDepositorInsertCre-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBI-GFP-Bub1-wt
Plasmid#16624DepositorInsertBUB1 (BUB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1(118-1274)
Plasmid#221207PurposeScChd1-aa118-1274 chromatin remodelerDepositorInsertScChd1 (aa118-1274)
UseTags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468PromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-EYFP
Plasmid#104388PurposePiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorInsertEnhanced Yellow Fluorescent Protein
UseCre/Lox; PiggybacTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2_RFP-60A
Plasmid#200394PurposePlasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.DepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Lyn11C32S-PIP4K2A
Plasmid#202748Purposeweakly plasma membrane targeted fluorescent protein-tagged enzymeDepositorInsertEGFP:LYN (1-11) (C3S):PIP4K2A (PIP4K2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
P1-EYFP-pA (Construct 5)
Plasmid#55197PurposeSynthetic promoter responsive to gRNA1DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2_Renilla_luc-60A
Plasmid#200397PurposeA plasmid IVT template containing a Renilla luciferase CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertRenilla luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-FlpERT2
Plasmid#149438PurposeROSA26 targetting vector with tamoxifen-inducible Flp recombinaseDepositorInsertFlp-ERT2 fusion protein
UseCre/Lox and Mouse TargetingTagsERT2ExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2_hMGFP-60A
Plasmid#200393PurposeA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertmonster GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
hHSF1 K80R
Plasmid#118350PurposePlasmid expresses hHSF1 with K80 in DBD mutated to R.DepositorInserthHSF1 (HSF1 Human)
UseTagsmyc and 6xHISExpressionMammalianMutationK80RPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh2-PH
Plasmid#71373PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh2-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa 277-398PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT3-FLAG-KLF3
Plasmid#49102PurposeExpresses KLF3 in mammalian cellsDepositorInsertKrüppel-Like Factor 3(KLF3)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Osh2-PH
Plasmid#71370Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertOsh2-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 277-398PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalB S28N
Plasmid#19722DepositorInsertV-ral simian leukemia viral oncogene homolog B (RALB Human)
UseRetroviralTagsExpressionMammalianMutationS28N for dominant negative function.PromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Num1-PH
Plasmid#71369Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertNum1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa2563-2692PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro RalB Q72L
Plasmid#19721DepositorInsertV-ral simian leukemia viral oncogene homolog B (RALB Human)
UseRetroviralTagsExpressionMammalianMutationQ72L constitutively active, GTPase DeficientPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh1-PH
Plasmid#71372PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa267-388PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK-Skm1-PH
Plasmid#71371Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertSkm1-PH
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa1-132PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only