Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,247 results for: Ado;

Showing: 3571 - 3600 of 9247 results

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TDP-43_CTD_N319A

Plasmid

#248873

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with N319A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_N319A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed N319A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M322A

Plasmid

#248874

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M322A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M322A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M322A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M323A

Plasmid

#248875

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M323A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M323A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M323A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_S317A

Plasmid

#248871

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with S317A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S317A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S317A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_I318A

Plasmid

#248872

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with I318A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_I318A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed I318A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_F313A

Plasmid

#248869

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with F313A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_F313A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed F313A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_F316A

Plasmid

#248870

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with F316A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_F316A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed F316A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_S342A

Plasmid

#248888

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with S342A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S342A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S342A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_M336A

Plasmid

#248884

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with M336A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M336A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M336A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_S333A

Plasmid

#248882

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with S333A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S333A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S333A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43_CTD_W334A

Plasmid

#248883

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with W334A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_W334A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed W334A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1015

Plasmid

#235704

Purpose

Expression of FL human VPS15-ZZ in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

VPS15 (PIK3R4 Human)

Tags

3xTEV-ZZ

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO350

Plasmid

#235717

Purpose

Expression of FL human VPS15 in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

VPS15 (PIK3R4 Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO1005

Plasmid

#235702

Purpose

Expression of FL human UVRAG in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

UVRAG (UVRAG Human)

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1274

Plasmid

#235709

Purpose

Expression of FL human VPS34 REIE > AAAA mutant in mammalian cells

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

VPS34 (PIK3C3 Human)

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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SRP19 (siRNA resistant) in pLX311

Plasmid

#233291

Purpose

Expression of WT SRP19 (siRNA resistant)

Depositor

Sefi Rosenbluh

Article

Xi et al Proc Natl Acad Sci U S A. 2025 Apr 15;12

Insert

SRP19 (SRP19 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Expresses WT SRP19 that is resistant to siRNA

Promoter

E1Fa

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-DHFR-3xFLAG

Plasmid

#217428

Purpose

Lentiviral overexpression of human DHFR

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-WIPI1promotor

Plasmid

#206840

Purpose

The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

Depositor

Tassula Proikas-Cezanne

Article

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

WIPI1 promotor (partial)

Use

Luciferase

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL-hygro-FAKS732A/S910A

Plasmid

#221642

Purpose

This retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (S732A/S910A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK double mutation S732A/S910A (dominant negativ…

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL-hygro-FAKP712/713A

Plasmid

#216544

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: P712/713A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (P712/713A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation P712/713A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL-hygro-FAKR177/178A

Plasmid

#216543

Purpose

This retroviral plasmid expresses a mutated cDNA (dominant negative: R177/178A) of human PTK2

Depositor

Georgia Konstantinidou

Article

Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

Insert

PTK2 (R177/178A) (PTK2 Human)

Use

Retroviral

Tags

No

Expression

Mammalian

Mutation

FAK dominant negative mutation R177/178A

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 _CTD_6F→A

Plasmid

#194269

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

F276A, F283A, F289A, F367A, F397A, F401A

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 _CTD_5M→A_CR

Plasmid

#194263

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M322A, M323A, M336A, M337A, M339A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 _CTD_5M→A_IDR

Plasmid

#194264

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307A, M311A, M359A, M405A, M414A mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 _CTD_5M→L_IDR

Plasmid

#194265

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307L, M311L, M359L, M405L, M414L mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 _CTD_5M→Y_IDR

Plasmid

#194266

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

M307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TDP-43 _CTD_5A→M_IDR

Plasmid

#194267

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

A297M, A367M, A381M, A388M, A391M mutations in TD…

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR201-Eps8L2_297-370

Plasmid

#196529

Purpose

Entry vector for Gateway with Eps8L2_297-370

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_297-370 (EPS8L2 Human)

Expression

Bacterial

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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