We narrowed to 7,296 results for: ef1a

1. pCF712

   Plasmid

   #121663

   Purpose

   U6-sgRNA EF1a-ProCas9-Flavi-P2A-Puro

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   Cas9(C)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL

   Plasmid

   #114373

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Kv4.2LL (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Kv4.2LL

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C

   Plasmid

   #114375

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Kv2.1C (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Kv2.1C

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C

   Plasmid

   #114372

   Purpose

   expresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartment

   Depositor

   Mingshan Xue

   Article

   Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

   Insert

   GtACR2-EYFP-Nlgn1C (NEWENTRY )
   

   Use

   AAV

   Tags

   EYFP and Nlgn1C

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. T2A Split/Link TurboID Kit

   Plasmid Kit

   #1000000249

   Purpose

   The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.

   Depositor

   Sven Eyckerman

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

6. pRDA_174

   Plasmid

   #136476

   Purpose

   Lentiviral expression of EnAsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   EnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-TurboCas-NLS-Blast

   Plasmid

   #232346

   Purpose

   Lentiviral expression vector for dCas9 fused to miniTurboNLS

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA, miniTurbo, V5 and 3xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. p65-mNeonGreen

   Plasmid

   #127172

   Purpose

   Fluorescently tagged p65 reporter

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   GFP-RelA (RELA Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXL004-ishRNA-scramble

   Plasmid

   #36311

   Depositor

   Sean Palecek

   Article

   Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

   Insert

   shRNA of scramble
   

   Use

   Lentiviral and RNAi

   Tags

   EF1a-TetR-IRES-RFP-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   TetO-H1

   Available since

   July 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti_MCP-p300_Hygro

    Plasmid

    #138459

    Purpose

    lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    p300 core
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_550

    Plasmid

    #203398

    Purpose

    All-in-one lentiviral expression of gRNA and EnAsCas12a

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Insert

    Enhanced Acidaminococcus Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cre Reporter

    Plasmid

    #62732

    Purpose

    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    LoxP-DsRED-STOP-LoxP-eGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    LoxP sites

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-Alpha

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-TO-ASCL1-DLX2

    Plasmid

    #182307

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    Ascl1 (Ascl1 Mouse)
    Dlx2 (Dlx2 Mouse)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and pTRE3G-bidirectional

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX307 LacZ

    Plasmid

    #117730

    Purpose

    Open reading frame vector encoding LacZ

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX311 Luciferase

    Plasmid

    #117735

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX307 Luciferase

    Plasmid

    #117734

    Purpose

    Open reading frame vector encoding Luciferase

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    firefly luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenti-mito-mTagBFP2

    Plasmid

    #216130

    Purpose

    Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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