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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,473 results for: pcDNA3.1+

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Showing: 2401 - 2420 of 9473 results

  1. pcDNA3.1-MS2-HA-HsDIS3_Z

    Plasmid
    #148111
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsDIS3 (DIS3 Human)
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148111-plasmid-map-sequence-id-407051

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  2. pcDNA3.1-MS2-HA-HshnRNPH1_Z

    Plasmid
    #148120
    Purpose
    Mammalian Expression of HshnRNPH1
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HshnRNPH1 (HNRNPH1 Human)
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the sequence give…
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148120-plasmid-map-sequence-id-396934

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  3. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid
    #181844
    Purpose
    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FluoSTEP-AKAR
    Tags
    mRuby2 and sfGFP(1-10)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181844-plasmid-map-sequence-id-364069

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  4. pcDNA3.1-MS2-HA-HshnRNPM_Z

    Plasmid
    #148126
    Purpose
    Mammalian Expression of HshnRNPM
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HshnRNPM (HNRNPM Human)
    Expression
    Mammalian
    Mutation
    two silent mutations compared to the sequenceof t…
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    148126-plasmid-map-sequence-id-357800

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  8. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid
    #126472
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Publication
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Or47a (Or47a Fly)
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126472-plasmid-map-sequence-id-246570

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  9. pcDNA3.1(+)-ExRai-AKAR(T/A)

    Plasmid
    #118406
    Purpose
    Negative-control mutant for ExRai-AKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR(T/A)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    118406-plasmid-map-sequence-id-242821

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  12. pcDNA3.1/Hygro(-)-B2-TS3

    Plasmid
    #104353
    Purpose
    Expresses human beta 2 integrin tension sensor
    Depositor
    Pontus Nordenfelt, Timothy Springer
    Publication
    Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human integrin b2 with tension sensor (ITGB2 Human)
    Expression
    Mammalian
    Mutation
    AA1-733 ITGB2 present
    Promoter
    CMV
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104353-plasmid-map-sequence-id-201133

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  13. pY126 (pcDNA3.1-huBoCpf1)

    Plasmid
    #92302
    Purpose
    Expression of humanized BoCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huBoCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92302-plasmid-map-sequence-id-181630

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  14. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid
    #92296
    Purpose
    Expression of humanized FbCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huFbCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92296-plasmid-map-sequence-id-181502

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  15. pY113 (pcDNA3.1-huPb2Cpf1)

    Plasmid
    #92272
    Purpose
    Expression of humanized Pb2Cpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huPb2Cpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92272-plasmid-map-sequence-id-181492

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  18. pcDNA3.1-nAChr-Alpha6

    Plasmid
    #48812
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    48812-plasmid-map-sequence-id-363332

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Showing: 2401 - 2420 of 9473 results