We narrowed to 24,590 results for: HAL;

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r

Plasmid

#162956

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

Plasmid

#162965

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r

Plasmid

#162965

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

His6-Halotag fusion tag with upstream E. coli ribosome binding site

Use

Synthetic Biology

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

Plasmid

#241142

Purpose

Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
tdMCP
tdPCP

Tags

(n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

Plasmid

#241142

Purpose

Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
tdMCP
tdPCP

Tags

(n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag

Plasmid

#238572

Purpose

Express NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF(RBD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag

Plasmid

#238572

Purpose

Express NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF(RBD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

Plasmid

#227158

Purpose

For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 and cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

Plasmid

#227164

Purpose

For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14, cysteine 82, and cysteine 43…

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

Plasmid

#227158

Purpose

For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

cyp-33e1 (cyp-33E1 Nematode)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 295 and cysteine 439 to alanine

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

Plasmid

#227164

Purpose

For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

Depositor

Yimon Aye

Article

Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

Insert

CYP2A6 (CYP2A6 Human)

Tags

Flag, HaloTag, and His6

Expression

Mammalian

Mutation

changed cysteine 14, cysteine 82, and cysteine 43…

Promoter

CMV and SP6

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, NanoLuc-KRAS WT-CRAF RBD-CRD-HaloTag

Plasmid

#236865

Purpose

Express NanoLuc(R)-KRAS WT-CRAF RBD-CRD-HaloTag(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
cRAF(RBD)-CRD (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NanoBRET BiBRET Vector, NanoLuc-KRAS WT-CRAF RBD-CRD-HaloTag

Plasmid

#236865

Purpose

Express NanoLuc(R)-KRAS WT-CRAF RBD-CRD-HaloTag(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
cRAF(RBD)-CRD (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NanoBRET BiBRET Vector, NanoLuc-NRAS (Q61K)-CRAF RBD-HaloTag

Plasmid

#236860

Purpose

Express NanoLuc(R)-NRAS (Q61K)-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

NRAS (NRAS Human)
cRAF(RBD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

Q61K

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge