We narrowed to 39,823 results for: Sis

1. pShuttle-Flag-XBP1u

   Plasmid

   #63679

   Purpose

   Adenoviral shuttle vector for XBP1u expression

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   unspliced

   Promoter

   CMV

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pMaRSC

   Plasmid

   #89189

   Purpose

   The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

   Depositor

   David Tirrell

   Article

   Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

   Insert

   Flag-L274G-T2A-mCherry (Mars1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1_SPTLC1_WT

   Plasmid

   #181920

   Purpose

   3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1

   Depositor

   Christian Metallo

   Article

   Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10

   Insert

   Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
   

   Use

   Lentiviral; Mammalian expression, , doxycycline i…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-PIF3-mEGFP

   Plasmid

   #100283

   Purpose

   Expresses PIF3 (1-100 aa) fused with mEGFP in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   Phytochrome interacting factor 3 (PIF3 Mustard Weed)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N-terminus (1-100 aa) of PIF3 is used

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_eA3A-NG-nCas9

   Plasmid

   #163536

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-GABRA1(A322D)-IRES2-EGFP

   Plasmid

   #170821

   Purpose

   human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

   Depositor

   Tingwei Mu

   Article

   Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

   Insert

   Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
   

   Use

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Mutation

   change Alanine 322 to Aspartate

   Promoter

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_Anc689-NG-nCas9

   Plasmid

   #163527

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

   Plasmid

   #175563

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL1
   

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   RUBY

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163533

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_UdgX-EE-UdgX-nCas9-UdgX

    Plasmid

    #163566

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJS647

    Plasmid

    #183176

    Purpose

    PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

    Depositor

    Timothy Whitehead

    Article

    Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

    Insert

    PYR1 (PYR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

    Plasmid

    #163543

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKT2/CAGXSP/PalmReNL

    Plasmid

    #182970

    Purpose

    Stably express PalmReNL in mammalian cells via the sleeping beauty transposon system.

    Depositor

    Masamitsu Kanada

    Article

    Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

    Insert

    PalmReNL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-mRIN2 dRA

    Plasmid

    #108941

    Purpose

    Mammalian expression of mRIN2 dRA

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RA domain (aa 751-836)

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-mRIN2 dRHVPS9

    Plasmid

    #108942

    Purpose

    Mammalian expression of mRIN2 dRHVPS9

    Depositor

    Guido Serini

    Article

    Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

    Insert

    Ras and Rab interactor 2 (Rin2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of RHVPS9 domain (aa 455-738)

    Promoter

    CMV

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Arf6 T27N- Venus in pcDNA3.1

    Plasmid

    #90464

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Article

    Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

    Insert

    Arf6 T27N (ARF6 )
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-bHs

    Plasmid

    #134259

    Purpose

    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

    Depositor

    Edwin Manuel

    Article

    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

    Insert

    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    codon optimized for Salmonella typhimurium

    Promoter

    AraC

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_UdgX-APOBEC1-UdgX-nCas9

    Plasmid

    #163555

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO V5 DNAJB1

    Plasmid

    #19522

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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