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pAc5.1B-lambdaN-HA-DmImp_B

Plasmid

#145941

Purpose

Insect Expression of DmImp

Depositor

Elisa Izaurralde

Insert

DmImp (Imp Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_1-127_A

Plasmid

#145901

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Publication

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_128-372_A

Plasmid

#145902

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Publication

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_1-127_A

Plasmid

#145903

Purpose

Insect Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Publication

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_1-127 (Dcp1 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_128-372_A

Plasmid

#145904

Purpose

Insect Expression of DmDCP1_128-372

Depositor

Elisa Izaurralde

Publication

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1_128-372 (Dcp1 Fly)

Expression

Insect

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Publication

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA[Tra].1026G

Plasmid

#112689

Purpose

express gRNA targeting Tra under dU6-3 promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 S1338A

Plasmid

#111808

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Publication

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (TSC2 Fly)

Tags

3xFlag

Expression

Insect

Mutation

S1338A

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-61

Plasmid

#92262

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Publication

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

NHA

Expression

Insect

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-59

Plasmid

#92261

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Publication

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Mutation

aa 940-1215

Promoter

Ac5

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_CUS1E07

Plasmid

#71086

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf13 (Taf13 Fly)

Use

Gateway Cloning

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C01

Plasmid

#70944

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc1 (Orc1 Fly)

Use

Gateway Cloning

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1C11

Plasmid

#70849

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc5 (Orc5 Fly)

Use

Gateway Cloning

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4H02

Plasmid

#71026

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Bre1 (Bre1 Fly)

Use

Gateway Cloning

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4A03

Plasmid

#71027

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

lds (lds Fly)

Use

Gateway Cloning

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4G04

Plasmid

#71038

Purpose

Gateway entry clone

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

polybromo (polybromo Fly)

Use

Gateway Cloning

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only