We narrowed to 2,498 results for: ERK

1. pRM055 His-TEV-Cas9 6xNLS 0C

   Plasmid

   #196246

   Purpose

   Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

   Plasmid

   #176531

   Purpose

   PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

   Depositor

   Jared Toettcher

   Article

   Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

   Inserts

   KGV transcription factor
   TagBFP
   

   Use

   Piggybac transposase integration vector

   Tags

   ErkKTR, Gal4 DNA binding domain, PEST destabiliza…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBC2T-Ypet-FtnA

   Plasmid

   #108969

   Purpose

   expresses BC2-tag-Ypet-FtnA in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   FtnA (ftnA Escherichia coli)
   

   Use

   Tags

   BC2-tag and Ypet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

   Plasmid

   #179527

   Purpose

   For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 K233A/K234A/K253A/K263A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed lysines 233, 234, 253, and 263 to alanine

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

   Plasmid

   #87776

   Purpose

   Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   MKK4 (MAP2K4 Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG RpH-LAMP1-3xFLAG

   Plasmid

   #163018

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EFNB1-bio-His

   Plasmid

   #51749

   Purpose

   Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   EFNB1 (EFNB1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1GFP/RNase H1 WT

   Plasmid

   #174447

   Purpose

   Express GFP-tagged RNase H1 WT in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. piRFP670-N1-GalT

   Plasmid

   #87325

   Purpose

   To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-EFNB2-RBD-Fc (WT)

    Plasmid

    #200978

    Purpose

    Mammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    IgG1 Fc

    Expression

    Mammalian

    Mutation

    Soluble Receptor-Binding Domain

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG R2pH-LAMP1-3xFLAG

    Plasmid

    #157940

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Chick beta actin pomoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF(UG) hSyn LAMP1-msGFP (JV012)

    Plasmid

    #179728

    Purpose

    Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    LAMP1 (Lamp1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONOR223 RpH-LAMP1-3xFLAG

    Plasmid

    #163019

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-myc-EFNB2 (WT)

    Plasmid

    #200975

    Purpose

    Mammalian expression plasmid for wild type, myc-tagged EFNB2

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EFNB2 (EFNB2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-His6-SUMO-MproC145A

    Plasmid

    #172653

    Purpose

    Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

    Depositor

    Stephen Harrison

    Article

    MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

    Insert

    SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis Tag and SUMO

    Expression

    Bacterial

    Mutation

    Catalytic Cysteine 145 mutated to Alanine

    Promoter

    T7

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pDONOR223 R2pH-LAMP1-3xFLAG

    Plasmid

    #157941

    Purpose

    gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Gateway system

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KAT6B-luciferase

    Plasmid

    #113353

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T-1 3xHA-MKK4-EE

    Plasmid

    #47577

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK4-EE (MAP2K4 Human)
    

    Use

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Lacks the first 36 residues of the RefSeq protein…

    Promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

20. T7pr-His6-MBP-TEV-dCas9-BirA*

    Plasmid

    #159994

    Purpose

    Bacterial expression of dCas9-BirA*

    Depositor

    Jacob Corn

    Article

    Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

    Insert

    dCas9-BirA*
    

    Use

    Tags

    6X-His, MBP, 3X-FLAG, NLS

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    T7

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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