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We narrowed to 7,057 results for: crispr cas9 plasmids

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Showing: 2441 - 2460 of 7057 results

  4. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-457690

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  5. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  6. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  9. pCJ1023

    Plasmid
    #228763
    Purpose
    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.
    Depositor
    Bradley Yoder
    Insert
    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228763-plasmid-map-sequence-id-455783

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  10. p35C-gRNA-ccdB

    Plasmid
    #246792
    Purpose
    Express gRNA in plant cells (protoplast or callus) for Cas9-mediated editing
    Depositor
    Caixia Gao
    Insert
    ccdB
    Use
    CRISPR
    Available Since
    March 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    246792-plasmid-map-sequence-id-490425

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  11. FD1

    Bacterial Strain
    #125258
    Purpose
    Derivative of MG1655 used for the screening of clones able to survive the bad seed toxicity of dCas9, while still efficiently silencing the expression of mcherry
    Depositor
    David Bikard
    Article
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Bacterial Resistance
    None
    Available Since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. pTOX.1.0-gDNA

    Plasmid
    #132470
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TOX (TOX Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132470-plasmid-map-sequence-id-267177

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  13. pZNF132.1.0-gDNA

    Plasmid
    #132468
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF132 (ZNF132 Human)
    Use
    CRISPR
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132468-plasmid-map-sequence-id-267133

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  14. pSOX13.1.0-gDNA

    Plasmid
    #132466
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    SOX13 (SOX13 Human)
    Use
    CRISPR
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132466-plasmid-map-sequence-id-267100

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  15. pPHTF2.1.0-gDNA

    Plasmid
    #132465
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    PHTF2 (PHTF2 Human)
    Use
    CRISPR
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132465-plasmid-map-sequence-id-267085

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  16. pRHOXF2B.1.0-gDNA

    Plasmid
    #132462
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RHOXF2B (RHOXF2B Human)
    Use
    CRISPR
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132462-plasmid-map-sequence-id-267036

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  17. pRFX7.1.0-gDNA

    Plasmid
    #132455
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RFX7 (RFX7 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132455-plasmid-map-sequence-id-266870

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  18. pZNF830.1.0-gDNA

    Plasmid
    #132450
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF830 (ZNF830 Human)
    Use
    CRISPR
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    132450-plasmid-map-sequence-id-266818

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  19. pZNF609.1.0-gDNA

    Plasmid
    #132447
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF609 (ZNF609 Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132447-plasmid-map-sequence-id-266793

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  20. pZNF780A.1.0-gDNA

    Plasmid
    #132446
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF780A (ZNF780A Human)
    Use
    CRISPR
    Available Since
    Dec. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132446-plasmid-map-sequence-id-266790

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Showing: 2441 - 2460 of 7057 results