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pAd-Track Flag HA PGC-1 alpha

Plasmid

#14426

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-Venus-Fluc (puro)

Plasmid

#140328

Purpose

Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Firefly Luciferase (luc2)

Use

Lentiviral

Promoter

human PGK promoter

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EFIa-iTGB1

Plasmid

#115799

Purpose

Integrin B1 overexpression

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

iTGB1 (ITGB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro

Plasmid

#31845

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

Mito (COX8A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pMSR:MSMEG_0709:Dendra

Plasmid

#159077

Purpose

T7 expression of C-term fluorescent fusion in E. coli

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0709

Use

Integrating mycobacterial expression vector attl5

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_0919:Dendra

Plasmid

#174936

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_0919

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR:MSMEG_5482:Dendra

Plasmid

#167437

Purpose

C-term fluorescent fusion

Depositor

Keith Derbyshire, Joseph Wade

Article

Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

Insert

MSMEG_5482

Use

Integrating mycobacterial expression vector attl5…

Tags

Gly-Ala Linker/Dendra2/1x Flag Tag

Expression

Bacterial

Promoter

pmycTetO

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pNK5785

Plasmid

#219753

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnLuz_v4 - tAOX

Use

Luciferase

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-A

Plasmid

#226407

Purpose

Expression of human VAP-A fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-A (VAPA Human)

Tags

T7-Xpress tag and mCherry

Expression

Mammalian

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pNK5712

Plasmid

#219752

Purpose

pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - nnH3H_v2 - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK5867

Plasmid

#219751

Purpose

pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - mcitHispS - tAOX

Expression

Yeast

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1508

Plasmid

#219750

Purpose

pGAP-like vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA under control of GAP promoter, for yeast expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pGAP - npgA - tAOX

Expression

Yeast

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc

Plasmid

#113862

Purpose

Triple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc

Use

Lentiviral

Expression

Mammalian

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-opEBNA2-IRES-mCherry

Plasmid

#220355

Purpose

Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Expression

Mammalian

Mutation

codon-optimised for expression in mammalian cells

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 WT

Plasmid

#186472

Purpose

Expression of human MOSPD2 fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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CHOP promoter (-649/+136) pmCherry-1

Plasmid

#36035

Depositor

Quan Lu

Article

Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

Insert

CHOP promoter (-647 to +136) (DDIT3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pmCherryC1 VAP-B

Plasmid

#226409

Purpose

Expression of human VAP-B fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-B (VAPB Human)

Tags

HA, T7-Xpress tag, and mCherry

Expression

Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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