We narrowed to 5,180 results for: pcdna3 1

1. pcDNA3-Xl-VSP1 R152Q GFP

   Plasmid

   #51884

   Purpose

   expression of Xl-VSP1 mutant fused to GFP in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)

   Plasmid

   #24653

   Depositor

   Jim Woodgett

   Article

   Yan et al Nature. . 372(6508):798-800.

   Insert

   SEK1 (MAP2K4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Ser220Ala, Thr224Leu

   Promoter

   Available since

   June 21, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3-HA-REDD1 85-232 (#427)

   Plasmid

   #99922

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal deletion; encodes AA 85-232

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-REDD1 96-110 (#189)

   Plasmid

   #99929

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

   Insert

   REDD1 (DDIT4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Internal deletion of AA 96-110

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mouse PKA-Calpha-mEGFP

   Plasmid

   #45521

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA catalytic subunit alpha tagged by mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3-mouse PKA-RIalpha-mEGFP

   Plasmid

   #45525

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RI alpha tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3-mouse PKA-RIIalpha-mEGFP

   Plasmid

   #45527

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RII alpha tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-mouse PKA-RIbeta-mEGFP

   Plasmid

   #45526

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RI beta tagged by mEGFP
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-rat PKA-RIIbeta-mEGFP

   Plasmid

   #45523

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA regulatory subunit RII beta tagged by mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-mouse PKA-Calpha-mPAGFP

    Plasmid

    #45524

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA catalytic subunit alpha tagged by mPAGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-MAVS-M1A-invitro(KaganE12)

    Plasmid

    #52016

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M1A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-MBP1-K295TAG-W340A-CAAX.pcDNA3-k

    Plasmid

    #127399

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon and W34…

    Promoter

    CMV

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FLAG-MBP1-E322TAG-E309C-W340A.pcDNA3-k

    Plasmid

    #127408

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, E309C …

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-MBP1-E322TAG-W340A-CAAX.pcDNA3-k

    Plasmid

    #127405

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon and W34…

    Promoter

    CMV

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-MAVS-M1A,M142A(KaganF62)

    Plasmid

    #52002

    Purpose

    Expresses the human MAVS CDS containing two point mutations M1A and M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala and Met 142 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid

    #181855

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Histidine 33 in DnaJB1 changed to glutamine.

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ERBB3-V5/HIS

    Plasmid

    #201104

    Purpose

    expression of human ERBB3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB3 receptor tyrosine kinase, full length, wildtype (ERBB3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Flag-IDH1

    Plasmid

    #62906

    Purpose

    Expresses wildtype IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-3XHA-p53 Δ1-42

    Plasmid

    #196269

    Purpose

    Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

    Depositor

    Laura Attardi

    Article

    Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

    Insert

    p53 Δ1-42 (Trp53 Mouse)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    deleted amino acids 1 to 42

    Promoter

    CMV

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3 Flag MKK7B2Jnk1a1

    Plasmid

    #19726

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1 (Map2k7, MAPK8 Human, Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge