We narrowed to 9,409 results for: try

1. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCI-neo:alpha4-mcherry

   Plasmid

   #45073

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   alpha4-mcherry (Chrna4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. ND4.2-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #179683

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1347A

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCMV-sp-HPV-TM(ICAM)

   Plasmid

   #196510

   Purpose

   Express HPV antigen epitope with ICAM transmembrane domain

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   a HPV epitope with CD8a stalk linker and ICAM TM domain
   

   Use

   Lentiviral

   Tags

   3xflag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx4-Rac1-2G

   Plasmid

   #66110

   Purpose

   Second generation Rac1 FRET biosensor

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET29b-pduCDE

   Plasmid

   #110138

   Purpose

   Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

   Insert

   pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ChromosomesMembranesandCytoskeleton

   Plasmid

   #62450

   Purpose

   MXS_chaining vector with 4 cassettes

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Inserts

   CMV::H2B-TagBFPx3-bGHpA
   CMV::Lyn-Ceruleanx3-bGHpA
   CMV::mCherryx3-linker-betaActin-bGHpA
   CMV::Citrinex3-linker-alphaTubulin-bGHpA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMVHA E2F1

   Plasmid

   #24225

   Purpose

   Mammalian expression of human E2F1 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Use

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET22b_PstS_3

    Plasmid

    #78200

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,I76G,A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17C,I76G,A197C (please see depositor comment bel…

    Promoter

    T7

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ220-Ultra

    Plasmid

    #176889

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    AsCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 612 p3E-2A-mCherryCAAXpA

    Plasmid

    #195971

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-mCherry-CAAX (membrane)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MT1E

    Plasmid

    #105702

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1e

    Depositor

    Artur Krężel

    Article

    Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

    Insert

    Metallothionein 1e (MT1E Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_HUWE1_isoform1

    Plasmid

    #190464

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET51b-His-TEV-SNAP-tag

    Plasmid

    #167269

    Purpose

    Overexpression of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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