Addgene

We narrowed to 13,544 results for: RING;

Showing: 1231 - 1240 of 13544 results

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pET51b-NAD-cpLuc2

Plasmid

#135629

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc2 sensor

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Use

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Promoter

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mUAVbla:tetR 5′

Plasmid

#149379

Purpose

mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Insert

tetA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLBH546_Tet-Cas14b1Locus

Plasmid

#112503

Purpose

Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLBH547_Tet-Cas14b2Locus

Plasmid

#112504

Purpose

Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14b2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSECRETS-A

Plasmid

#196986

Purpose

For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

Depositor

Eric Josephs

Article

Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pltetO1

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-cpLuc

Plasmid

#135628

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V1)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc sensor

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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stathmin-CFP

Plasmid

#86783

Purpose

Mammalian expression of stathmin-CFP

Depositor

Lynne Cassimeris

Article

Ringhoff et al Mol Biol Cell. 2009 Aug;20(15):3451-8. d

Insert

stathmin (STMN1 Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-Luc

Plasmid

#135627

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-Luc sensor

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169

Plasmid

#209796

Purpose

Bacterial expression of human PRL-3 without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2004 Mar 19. 279(12):11882-

Insert

PRL-3 (PTP4A3 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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