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pAAV-EFS-hfCas13d-T2A-mCherry-pA

Plasmid

#192497

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGgaatacattaccaacGTGGTGtacGTG

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA

Plasmid

#192483

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.4CaMKIIa

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CasRx-T2A-mCherry-pA

Plasmid

#192484

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

Ef1a

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-cOpn5-T2A-mcherry

Plasmid

#237857

Purpose

The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of a EF1α promoter.

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV

Promoter

EF1α

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSA358_pAAV-SCP1-Intron-eGFP-CS1

Plasmid

#215513

Purpose

Single stranded AAV eGFP reporter vector with SCP1 promoter.

Depositor

Stein Aerts

Article

Hecker et al Science. 2025 Jan 2;387(6735):eadp3957.

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

SCP1

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV TRE-Tight CheRiff-mKate2

Plasmid

#182493

Purpose

Expression of CheRiff channelrhodopsin linked to mKate2 from a Tet-inducible promoter

Depositor

Thomas Oertner

Article

Lamothe-Molina et al Nat Commun. 2022 Oct 26;13(1):6376. doi:

Insert

CheRiff (#51697 Adam Cohen Lab)

Use

AAV

Tags

mKate2 (Evrogen)

Expression

Mammalian

Promoter

TRE-Tight (Clontech)

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-TdTomato-WPRE-polyA

Plasmid

#241828

Purpose

AAV construct for constitutive expression of TdTomato.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

TdTomato-WPRE-polyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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rAAV-Ef1α-EGFP-WPRE-ID-PolyA

Plasmid

#241829

Purpose

AAV construct for constitutive expression of EGFP. Contains a molecular barcode for identification of AAV genome using single-cell sequencing.

Depositor

Benjamin Arenkiel

Article

Belfort et al Front Neurosci. 2025 Feb 13;19:1531122.

Insert

EGFP-WPRE-ID-PolyA

Use

AAV

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-cOpn5-T2A-mcherry

Plasmid

#237860

Purpose

The transfer plasmid for packaging AAV expressing chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of GfaABC1D promoter

Depositor

Minmin Luo

Article

Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

Insert

Opn5 (OPN5 Chicken)

Use

AAV

Promoter

GfaABC1D

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CMV-RFP-p2A-DAAO-NES

Plasmid

#238920

Purpose

Expression of DAAO with a nuclear export signal and RFP as a reporter in mammalian cells

Depositor

Vsevolod Belousov

Article

Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

Insert

RFP-p2A-DAAO-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-AscI-CAG-H2B-mBaoJin

Plasmid

#229576

Purpose

Expresses H2B-mBaoJin in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Use

AAV

Expression

Mammalian

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-AscI-CAG-dMito2-mBaoJin

Plasmid

#229575

Purpose

Expresses COX8A-mBaoJin fusion in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Use

AAV

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeonGreen-P2A-FusionRed

Plasmid

#229570

Purpose

Expresses mNeonGreen and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-P2A-FusionRed

Plasmid

#229583

Purpose

Expresses EGFP and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

EGFP

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mGreenLantern-P2A-FusionRed

Plasmid

#229584

Purpose

Expresses mGreenLantern and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mGreenLantern

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-td8ox2StayGold(c4)-P2A-FusionRed

Plasmid

#229585

Purpose

Expresses td8ox2StayGold(c4) and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

td8ox2StayGold

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-AausFP1-P2A-FusionRed

Plasmid

#229586

Purpose

Expresses AausFP1 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

AausFP1

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mClover3-P2A-FusionRed

Plasmid

#229592

Purpose

Expresses mClover3 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mClover3

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-StayGold-P2A-mCherry

Plasmid

#229599

Purpose

Expresses StayGold and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

StayGold

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mGreenLantern-P2A-mCherry

Plasmid

#229605

Purpose

Expresses mGreenLantern and mCherry in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mGreenLantern

Use

AAV

Expression

Mammalian

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-ExRai-AKAR2-T/A

Plasmid

#171847

Purpose

Cre-dependent, synapsin promoter-driven expression of the negative control phosphomutant ExRai-AKAR2 T/A PKA biosensor in neurons.

Depositor

Richard Huganir

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2 T/A

Use

AAV

Expression

Mammalian

Mutation

T6A phospho-deficient mutation

Promoter

hSyn1

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec

Plasmid

#239596

Purpose

expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

Plasmid

#239594

Purpose

expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

Plasmid

#239595

Purpose

expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

Depositor

Andreas Moor

Article

Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

Insert

Plxnb2 (Plxnb2 Mouse)

Use

AAV

Expression

Mammalian

Promoter

hU6

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon1-WPRE

Plasmid

#240053

Purpose

Constitutive expression of genetically encoded voltage indicator Ace-mNeon1

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

Ace-mNeon1

Use

AAV

Promoter

CaMKII

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-Varnam1-WPRE

Plasmid

#240054

Purpose

Cre dependent Red fluorescent voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM1

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-Varnam2-WPRE

Plasmid

#240055

Purpose

Cre dependent Red fluorescent, negative response-polarity voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM2

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-RandE3-P2A-GFP

Plasmid

#236181

Purpose

AAV version of constitutive control plasmid

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

RandE3-P2A-GFP

Use

AAV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-MCS-noATG-GFP

Plasmid

#223181

Purpose

A multicloning site (MCS) was synthesized and inserted into AAV-CMV-GFP to construct the AAV-CMV-MCS-(no ATG)-GFP negative control plasmid.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Type

Empty backbone

Use

AAV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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