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pMRMTK-clo24

Plasmid

#203994

Purpose

Cloning plasmid containing the Hro ES5 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES5

Expression

Plant

Promoter

Hro ES5

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pNK1198

Plasmid

#219740

Purpose

MoClo-compatible Level 0 - 3U + terminator vector carrying terminator HSP18.2

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

The heat shock protein 18.2 (HSP) terminator

Use

Synthetic Biology

Expression

Plant

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo25

Plasmid

#203995

Purpose

Cloning plasmid containing the Hro ES6 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES6

Expression

Plant

Promoter

Hro ES6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo23

Plasmid

#203993

Purpose

Cloning plasmid containing the Hro ES4 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES4

Expression

Plant

Promoter

Hro ES4

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-SHmOCR

Plasmid

#206874

Purpose

Empty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusion

Depositor

Murray Junop

Article

Szabla et al Nucleic Acids Res. 2024 Jul 22:gkae635.

Type

Empty backbone

Tags

HSV + 6xHis and SuperHis (14xHis) + monomeric OCR…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pACBE-ASR (pXK-607)

Plasmid

#208822

Purpose

Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Promoter

CMV, U6

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pACBE-FSR (pXK-1648)

Plasmid

#208821

Purpose

Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

Depositor

Kun Xu

Article

Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

Type

Empty backbone

Tags

EGFP

Expression

Mammalian

Promoter

CMV, U6

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo39

Plasmid

#204014

Purpose

Cloning plasmid containing the sfGFP insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

super folder green fluorescent protein

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo4

Plasmid

#203925

Purpose

Cloning plasmid containing the R6Kγ origin and oriT insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

R6Kγ origin of replication and oriT

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo11

Plasmid

#203981

Purpose

Cloning plasmid containing the Lambda tl3 terminator insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Lambda tl3 Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo12

Plasmid

#203982

Purpose

Cloning plasmid containing the rrnB T1 terminator insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

rrnB T1 Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo13

Plasmid

#203983

Purpose

Cloning plasmid containing the tonB & SoxR terminators insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

tonB & SoxR Terminator

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo14

Plasmid

#203984

Purpose

Cloning plasmid containing the Elu ES1 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES1

Expression

Plant

Promoter

Elu ES1

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo16

Plasmid

#203986

Purpose

Cloning plasmid containing the Elu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Elu ES3

Expression

Plant

Promoter

Elu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo18

Plasmid

#203988

Purpose

Cloning plasmid containing the Ppu ES2 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES2

Expression

Plant

Promoter

Ppu ES2

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo19

Plasmid

#203989

Purpose

Cloning plasmid containing the Ppu ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ppu ES3

Expression

Plant

Promoter

Ppu ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo22

Plasmid

#203992

Purpose

Cloning plasmid containing the Hro ES3 insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Hro ES3

Expression

Plant

Promoter

Hro ES3

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo1

Plasmid

#203919

Purpose

Cloning plasmid containing the BHR1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

BHR1 Origin of Replication

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Tornado-RNA sensor for survivin

Plasmid

#185405

Purpose

circular RNA-scaffolded sensing modules for survivin

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

circular RNA-scaffolded sensing modules

Expression

Mammalian

Mutation

WT

Promoter

U6

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e VPR

Plasmid

#188515

Purpose

Expresses FLAG tagged DpbdCas12e VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DpbCas12e

Plasmid

#188495

Purpose

Expresses FLAG tagged DpbCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PlmCas12e

Plasmid

#188496

Purpose

Expresses FLAG tagged PlmCas12e

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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DpbdCas12e KRAB

Plasmid

#188505

Purpose

Expresses FLAG tagged DpbdCas12e KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12e

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D672A/E769A/D935A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Cas12j3

Plasmid

#188497

Purpose

Expresses FLAG tagged Cas12j3

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_ABE‐NG‐loop

Plasmid

#187057

Purpose

ABEmax-SpCas9-NG-loop

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

ABE‐NG‐loop

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBlueScript II SK-SNAP donor for Hist1h3g

Plasmid

#186932

Purpose

Genomic targeting of SNAP tag at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

SNAP donor for Hist1h3g (H3c8 Mouse)

Tags

SNAP

Expression

Mammalian

Promoter

T7

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole4 KO sgRNA

Plasmid

#186935

Purpose

Pole4 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole4 KO sgRNA (Pole4 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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px459-Pole3 KO sgRNA

Plasmid

#186934

Purpose

Pole3 KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Pole3 KO sgRNA (Pole3 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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L0-AA008

Plasmid

#185754

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis EF1a promoter (pAaEF1a).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaEF1a (promoter)

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L0-AA018

Plasmid

#185753

Purpose

Level 0 loop assembly vector containing the Anthoceros agrestis Tip1;1 promoter (pAaTip1;1).

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Insert

pAaTip1;1 (promoter)

Use

Synthetic Biology

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BicC.Ca9.R

Plasmid

#168295

Purpose

Expresses Cas9 in Drosophila late germline cells

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

BicC.Cas9-T2A-eGFP-p10

Use

CRISPR

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261

Plasmid

#171434

Purpose

Expression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

tdMCP2-GyG-(GGS)X6-HaloTag C261

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-AtTPR2(1-209)

Plasmid

#177860

Purpose

Bacterial Expression of TPR2

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR2 (TPR2 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcry:mCherry,-600unc:cct3GFP

Plasmid

#172528

Purpose

alphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.

Depositor

Joachim Berger

Article

Berger et al Cell Rep. 2018 Jan 9;22(2):313-322. doi:

Insert

cct3-GFP

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-zfTpo

Plasmid

#168198

Purpose

Express zebrafish thrombopoietin (Tpo), mRNA synthesis

Depositor

Petr Bartunek

Article

Svoboda et al Blood. 2014 Jul 10;124(2):220-8. doi: 10

Insert

Thrombopoietin (thpo Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1-Rnt1-dsRBD

Plasmid

#171549

Purpose

Encoding part of the budding yeast Rnt1 protein from L366 to S453

Depositor

Yan Zeng

Article

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Rnt1 (RNT1 Budding Yeast)

Expression

Bacterial

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PHY55-mouse U6-sgLMNA

Plasmid

#164045

Purpose

U6-driven sgRNA targeting LMNA

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

LMNA targeting sgRNA

Use

CRISPR

Promoter

mouse U6

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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Adeno-miR-23b

Plasmid

#112538

Purpose

Adenovirus overexpression

Depositor

Shusheng Wang

Article

Zhou et al Proc Natl Acad Sci U S A. 2011 May 17;10

Insert

miR-23b (MIR23B Human)

Use

Adenoviral

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-161K enhancer

Plasmid

#138365

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence.

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161k human enhancer

Use

Gateway compatible

Mutation

9 A bases deletion, that have no impact on the en…

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4921 - 4960 of 32585 results

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