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pFusX4-31

Plasmid

#63440

Purpose

Encodes RVD positions 10-12 in TALEN assembly

Depositor

Stephen Ekker

Article

Ma et al Hum Gene Ther. 2016 Jun;27(6):451-63. do

Insert

HD NG NN

Use

TALEN

Mutation

none

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
5mCUC1 pBluescript

Plasmid

#12065

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Expression

Bacterial

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
Syn3-27-146+TM

Plasmid

#14679

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector; aa 27-14…

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab1 WT

Plasmid

#37681

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab1 (Rab1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab3 WT

Plasmid

#44820

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab3 (Rab3 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab14 WT

Plasmid

#37714

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab14 (Rab14 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2A

Plasmid

#13473

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-46

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2B

Plasmid

#13474

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2B (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 47-136

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A

Plasmid

#13476

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L17A

Plasmid

#13477

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L17A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 17 to Alanine

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
Syn3D38

Plasmid

#13397

Depositor

Thomas Weimbs

Article

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3D38 (STX3 Human)

Tags

2xMyc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. First N-…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pConnector_L7-RE

Plasmid

#254032

Purpose

A plasmid containing the L7-RE connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L7-spacer-RE

Use

Synthetic Biology

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L4-RE

Plasmid

#254028

Purpose

A plasmid containing the L4-RE connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L4-spacer-RE

Use

Synthetic Biology

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L6-R7

Plasmid

#254030

Purpose

A plasmid containing the L6-R7 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L6-spacer-R7

Use

Synthetic Biology

Available Since

April 29, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_LS-R1

Plasmid

#254022

Purpose

A plasmid containing the LS-R1 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

LS-spacer-R1

Use

Synthetic Biology

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pConnector_L2-RE

Plasmid

#254025

Purpose

A plasmid containing the L2-RE connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L2-spacer-RE

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L7-R8

Plasmid

#254031

Purpose

A plasmid containing the L7-R8 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L7-spacer-R8

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L2-R3

Plasmid

#254024

Purpose

A plasmid containing the L2-R3 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L2-spacer-R3

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L5-R6

Plasmid

#254029

Purpose

A plasmid containing the L5-R6 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L5-spacer-R6

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L1-R2

Plasmid

#254023

Purpose

A plasmid containing the L1-R2 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L1-spacer-R2

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L8-RE

Plasmid

#254033

Purpose

A plasmid containing the L8-RE connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L8-spacer-RE

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L3-R4

Plasmid

#254026

Purpose

A plasmid containing the L3-R4 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L3-spacer-R4

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pConnector_L4-R5

Plasmid

#254027

Purpose

A plasmid containing the L4-R5 connector.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

L4-spacer-R5

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
EBFP2-Kindlin2-3KA

Plasmid

#242823

Purpose

Encodes human kindlin2-3KA mutant and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

K385A, K386A, K393A

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-Kindlin2

Plasmid

#242822

Purpose

Encodes wildtype human kindlin2 and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

N/A

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-K1K2

Plasmid

#242819

Purpose

Encodes human kindlin1 containing kindlin2 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin1 (Fermt1 Human)

Expression

Mammalian

Mutation

Kindlin1 chimera containing the F3 domain of kind…

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-3AF

Plasmid

#245785

Purpose

Expression of mutated bovine arrestin-3 (I385A, V386A, F387A) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

I385A, V386A, F387A

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LEU3_SIR4

Plasmid

#190582

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LEU3 (LEU3 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LYS14_SIR4

Plasmid

#190583

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LYS14 (LYS14 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADH1pro_YRR1_SIR4

Plasmid

#190585

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

YRR1 (YRR1 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADH1pro_TEA1_SIR4

Plasmid

#190586

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

TEA1 (TEA1 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pZNpF-∆7

Plasmid

#245367

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆7 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

7-bp deletion, H96Vfs*29

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pZNpF-∆8

Plasmid

#245368

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆8 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

synthetic 8-bp deletion

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pZNpF-∆59

Plasmid

#245365

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆59 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

59-bp deletion, D78Vfs*6

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pZNpF-∆11

Plasmid

#245366

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆11 (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mE-mCherry2A

Plasmid

#236248

Purpose

Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately

Depositor

James Amatruda, Genevieve Kendall

Article

Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10

Insert

mCherry2A

Expression

Mammalian

Mutation

Added a viral2A sequence to mCherry with no stop …

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241137

Purpose

Expresses Anti-GCN4 scFv-sfGFP and HaloTag-tdMCP to track mature and nascent SunTag-tagged proteins and MS2 tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-GCN4 scFv
tdMCP

Tags

HaloTag and sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCAGGS-CIBN-p53CT(98-393)-mNeonGreen

Plasmid

#241844

Purpose

Expresses a localizer of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CIBN and mNeonGreen

Expression

Mammalian

Mutation

C-terminus (97-393 aa)

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Integrin β3-EBFP2

Plasmid

#242827

Purpose

Encodes wildtype human integrin β3 and C-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 3 (ITGB3 Human)

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
F-IKKε v2

Plasmid

#169773

Purpose

Express IKKε v2 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2

Tags

Flag tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-29_GSS-6xHis-SUMO-VidC-EGFP-FLAG

Plasmid

#241009

Purpose

VidC-EGFP construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGFP-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-29_GSS-6xHis-SUMO-VidC-mCherry-FLAG

Plasmid

#241010

Purpose

VidC-mCherry construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-mCherry-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21_GSS-6xHis-SUMO-VidC-bla-FLAG

Plasmid

#241012

Purpose

VidC-bla construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-bla-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21_GSS-6xHis-SUMO-VidC-EGF-FLAG

Plasmid

#241011

Purpose

VidC-EGF construct that can be purified via His-tag and undergo N-terminal conjugation using the VEPL system.

Depositor

Cole DeForest

Article

Gharios et al Bioconjug Chem. 2024 Jun 19;35(6):750-75

Insert

GSS-6xHis-SUMO-VidC-EGF-FLAG

Tags

FLAG-tag, His-tag, and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-HRP-TM

Plasmid

#231497

Purpose

Expressing HRP on the cell surface of mammalian cells

Depositor

Baljit Khakh

Article

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG HRP-TM

Plasmid

#231501

Purpose

Expressing HRP on the cell surface under CAG promoter

Depositor

Baljit Khakh

Article

Wu et al Neuron. 2025 Jun 5:S0896-6273(25)00366-6

Insert

HRP

Use

AAV

Tags

Igk, HA, Myc, PDGFR-beta transmembrane domain

Expression

Mammalian

Promoter

CAG

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pVEA3-delta7

Plasmid

#234802

Purpose

Mammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 176, 177, 178, 182-185

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IP DEPP1-mCherry

Plasmid

#224559

Purpose

Expresses DEPP1 fused to mCherry in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IP DEPP1 dTSnare-mCherry

Plasmid

#224561

Purpose

Expresses DEPP1 dTsnare fused to mCherry in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

DEPP1 (DEPP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

removed Tsnare domain

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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