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Showing: 6151 - 6200 of 19729 results

  1. pCm N-term MBP SMT3 NikJC199U

    Plasmid
    #174363
    Purpose
    Expression plasmid with a ULP1 removable N-term MBP SUMO tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Tags
    8xHis, MBP, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available Since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174363-plasmid-map-sequence-id-342843

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  6. KG#920

    Plasmid
    #110937
    Purpose
    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110937-plasmid-map-sequence-id-212471

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  7. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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  8. pGBW-m4046774

    Plasmid
    #145574
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145574-plasmid-map-sequence-id-287751

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  9. pGBW-m4046846

    Plasmid
    #145575
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145575-plasmid-map-sequence-id-287752

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  25. pVR2-m4

    Plasmid
    #84723
    Purpose
    pVR2 but with G to U and A to C mutations following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    G to U mutation at the first nucleotide after the…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84723-plasmid-map-sequence-id-167501

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  26. pVR2-m5

    Plasmid
    #84724
    Purpose
    pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    insertion of 1 U between positions 39 and 40 of a…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84724-plasmid-map-sequence-id-167505

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  27. pVR2-m6

    Plasmid
    #84725
    Purpose
    pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    insertion of 2 Us between positions 39 and 40 of …
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84725-plasmid-map-sequence-id-167510

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  28. pVR2-m7

    Plasmid
    #84726
    Purpose
    pVR2 but with a G to U mutation immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    G to U mutation at position 41 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84726-plasmid-map-sequence-id-167513

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  29. pVR2-m8

    Plasmid
    #84727
    Purpose
    pVR2 but with two G to U mutations immediately following the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    G to U mutations at positions 40 and 41 of annota…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84727-plasmid-map-sequence-id-167518

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  30. pVR2-m1

    Plasmid
    #84720
    Purpose
    pVR2 but with a C to U mutation in the riboswitch aptamer
    Depositor
    Nils Walter
    Article
    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    C to U mutation at position 13 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    84720-plasmid-map-sequence-id-167487

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  32. pCEP4-SERTR596Q-myc

    Plasmid
    #107483
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R596Q conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    Y289I
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107483-plasmid-map-sequence-id-202737

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  34. pCEP4-SERTK605N-myc

    Plasmid
    #107475
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K605N approxmately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    K605N
    Promoter
    CMV
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    107475-plasmid-map-sequence-id-202729

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  35. pCEP4-SERTF465L-myc

    Plasmid
    #107469
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F465L conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    F465L
    Promoter
    CMV
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    107469-plasmid-map-sequence-id-207197

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  36. pCEP4-SERTR462E-myc

    Plasmid
    #107489
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation R462E with approximately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    R462E
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107489-plasmid-map-sequence-id-205681

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  37. pCEP4-SERTG582K-myc

    Plasmid
    #107497
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G582K conferring gain of function phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    G582K
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107497-plasmid-map-sequence-id-205726

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  38. pCEP4-SERTI576K-myc

    Plasmid
    #107496
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I576K with approximately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    I576K
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107496-plasmid-map-sequence-id-205718

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  39. pCEP4-SERTF556H-myc

    Plasmid
    #107495
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F556H conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    F556H
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107495-plasmid-map-sequence-id-205714

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  40. pCEP4-SERTP499K-myc

    Plasmid
    #107494
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation P499K conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    P499K
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107494-plasmid-map-sequence-id-205711

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  41. pCEP4-SERTE494Y-myc

    Plasmid
    #107493
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation E494Y conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    E494Y
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107493-plasmid-map-sequence-id-205709

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  42. pCEP4-SERTK490L-myc

    Plasmid
    #107492
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation K490L conferring gain of function phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    K490L
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107492-plasmid-map-sequence-id-205701

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  43. pCEP4-SERTL467D-myc

    Plasmid
    #107490
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation L467D with approximately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    L467D
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107490-plasmid-map-sequence-id-205686

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  44. pCEP4-SERTC473H-myc

    Plasmid
    #107491
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation C473H conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    C473H
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107491-plasmid-map-sequence-id-205691

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  45. pCEP4-SERTY410G-myc

    Plasmid
    #107488
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation Y410G with approximately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    Y410G
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107488-plasmid-map-sequence-id-205679

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  46. pCEP4-SERTI379Q-myc

    Plasmid
    #107485
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I379Q conferring gain of function phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    I379Q
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107485-plasmid-map-sequence-id-205663

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  47. pCEP4-SERTV343T-myc

    Plasmid
    #107484
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation V343T conffering gain of function phenotype, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    V343T
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107484-plasmid-map-sequence-id-205658

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  48. pCEP4-SERTF263C-myc

    Plasmid
    #107482
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F263C with approximately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    F263C
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107482-plasmid-map-sequence-id-205653

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  49. pCEP4-SERTA173G-myc

    Plasmid
    #107479
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation A173G with approximately wildtype activity, decreased surface expression
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    A173G
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107479-plasmid-map-sequence-id-205640

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  50. pCEP4-SERTF88H-myc

    Plasmid
    #107478
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation F88H conferring gain of function phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Expression
    Mammalian
    Mutation
    F88H
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107478-plasmid-map-sequence-id-205623

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Showing: 6151 - 6200 of 19729 results