We narrowed to 18,031 results for: Mut

1. Hs.KRAS4b M188L

   Plasmid

   #83141

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), M188L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   M188L

   Promoter

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK324

   Plasmid

   #71838

   Purpose

   Cloned region contains Acetobacter aceti 1023 purE gene

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK340

   Plasmid

   #71837

   Purpose

   Cloned region contains Acetobacter aceti 1023 purE(H59N) gene

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to asparagine

   Promoter

   lac

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK346

   Plasmid

   #71836

   Purpose

   Cloned region contains Acetobacter aceti 1023 purE(H59D) gene

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changes histidine-59 to aspartate

   Promoter

   lac

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJBL-RNAINS4

   Plasmid

   #69456

   Purpose

   RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S4 + SFGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   mutations for S4 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL-RNAINS3

   Plasmid

   #69455

   Purpose

   RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   mutations for S3 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available Since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTH1-SaSrtA-A

   Plasmid

   #64979

   Purpose

   Expresses the E108A mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108A

   Promoter

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH1-SaSrtA-K

   Plasmid

   #64978

   Purpose

   Expresses the E105K mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E105K

   Promoter

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

   Plasmid

   #52007

   Purpose

   Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   5utr-MAVS-ORF2,3mut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutated start codons for ORF3 and 4

   Promoter

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pRS313-prp8-S2197F

    Plasmid

    #89967

    Purpose

    S2197F point mutant

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S2197F

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS313-prp8-H2387P

    Plasmid

    #89968

    Purpose

    H2387P point mutant

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H2387P

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS313-prp8-R2388G

    Plasmid

    #89969

    Purpose

    R2388G point mutant

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R2388G

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT Draper-delta ITAM-GFP

    Plasmid

    #126655

    Purpose

    Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper with ITAM Tyr residues mutated to Ala
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Cytoplasmic ITAM Tyr residues mutated to Phe

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His10-Draper delta ITAM

    Plasmid

    #126671

    Purpose

    Expression of His10-Draper delta ITAM mutant for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic ITAM residues mutated to Phe
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cytoplasmic ITAM residues mutated to Phe

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLT3-ITD-MSCV-IRES-GFP

    Plasmid

    #184779

    Purpose

    Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

    Depositor

    Timothy Ley

    Insert

    FLT3 (FLT3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Activating internal tandem duplication (ITD) adde…

    Promoter

    MSCV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-neo FlucDM EGFP

    Plasmid

    #90172

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R188Q, R261Q in FLuc

    Promoter

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK1043

    Plasmid

    #8835

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    tobacco etch virus protease, catalytic domain
    

    Use

    Tags

    MBP and polyarginine

    Expression

    Bacterial

    Mutation

    Autolysis-resistant S219V mutant

    Promoter

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAR-Ec633

    Plasmid

    #130873

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L477V, L640Y, I777K, W814N

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Promoter

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRRL_U2AF1_S34F_mCherry

    Plasmid

    #84019

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34F

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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