We narrowed to 11,540 results for: Vars;

1. AAV-oChIEF-tdTomato

   Plasmid

   #50977

   Purpose

   AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mutation

   ChIEF gene is mammalian codon optimized

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. ttAtCas12a+int

   Plasmid

   #182384

   Purpose

   Gives very high editing efficiency in barley. Also works in Brassica oleracea

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   LbCas12a coding sequence with D156R and Arabidopsis introns
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R + 8 Arabidopsis introns added

   Promoter

   None

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBLego

   Plasmid

   #219967

   Purpose

   The pSBLego vector enables sybodies for the Legobody method with a PelB signal for periplasmic expression and a "SLEHHHHHH" motif for purification and Legobody Fab binding.

   Depositor

   Markus Seeger

   Article

   Ackle et al Sci Rep. 2025 Mar 19;15(1):9460. doi: 10

   Type

   Empty backbone

   Use

   Tags

   SLEHHHHHH and pelB signal sequence

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-11-LECT2-I40

   Plasmid

   #183032

   Purpose

   Expression of LECT2 I40 variant protein from T7 promoter

   Depositor

   Gareth Morgan

   Article

   Belonogov et al bioRxiv 2022.03.01.482540

   Insert

   LECT2 (LECT2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I40 variant

   Promoter

   T7

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD441_WT_hi

   Plasmid

   #128857

   Purpose

   High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

   Depositor

   Mark Pallen

   Article

   Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

   Insert

   Flagellin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

   Plasmid

   #175534

   Purpose

   Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag7-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-Core Traptavidin

   Plasmid

   #26054

   Depositor

   Mark Howarth

   Article

   Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

   Insert

   Core Traptavidin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Streptavidin with 2 amino acid mutations: S52G R5…

   Promoter

   Available Since

   Aug. 30, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pBS-HxB.S

   Plasmid

   #65415

   Purpose

   contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

   Depositor

   Harold Erickson

   Article

   Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

   Insert

   tenascin C small splice variant (TNC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-Vinexin-C-14

   Plasmid

   #54305

   Purpose

   Localization: Focal Adhesions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Vinexin (SORBS3 Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pcDNA5/FRT/TO_NES_HaloTag9

    Plasmid

    #175537

    Purpose

    Strict cytosolic expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    NES-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EEF1A2 in pENTR

    Plasmid

    #216585

    Purpose

    gateway cloning donor vector containing EEF1A2

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    EEF1A2 (EEF1A2 Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT3928

    Plasmid

    #113331

    Purpose

    control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-Cas9

    Plasmid

    #64237

    Purpose

    expresses codon optimized Cas9 in zebrafish

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid

    #230056

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. SaABEmax

    Plasmid

    #119814

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSaCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. epB_CAG_TetRFlag-nls-tdTom_DEx4

    Plasmid

    #119909

    Purpose

    ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    TetRFlag-nls-tdTom
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GeNL(Ca2+)_520/pcDNA3

    Plasmid

    #85204

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_520
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. DDX3X in pENTR

    Plasmid

    #216572

    Purpose

    gateway cloning donor vector containing DDX3X

    Depositor

    Douglas Allan, Christopher Loewen

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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