We narrowed to 7,296 results for: ef1a

1. JD226

   Plasmid

   #229484

   Purpose

   Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k genes

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E2A, VA RNA I, L4-22k
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (E2A), Ef1a (L4-22k) and wt promoter (VA RNA …

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1302-AAV-EFSNC-dCjCas9-KRAB

   Plasmid

   #223147

   Purpose

   Expression of KRAB with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1312-AAV-EFSNC-dSaCas9-KRAB

   Plasmid

   #223157

   Purpose

   Expression of KRAB with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS-HsProm1s1(W795R)-StayGold

   Plasmid

   #211334

   Purpose

   Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Prominin 1 (PROM1 Human)
   

   Use

   AAV

   Tags

   StayGold

   Expression

   Mutation

   Changed Trp-795 to Arg

   Promoter

   EF1a

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

   Plasmid

   #207171

   Purpose

   expression plasmid for Gal4DBD-HIF-CAD.myc expression

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   Gal4DBD-HIF1a-CAD Myc tag linker
   

   Use

   Tags

   2xMyc tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-Puro_CI-hCOL4A5-Nluc_R1683X

   Plasmid

   #198111

   Purpose

   lentiviral expression of hCOL4A4_R1683Xin mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Lentiviral

   Tags

   Nluc

   Expression

   Mutation

   R1683X

   Promoter

   EF1a

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-Puro_CI-hCOL4A5-Nluc_C1684X

   Plasmid

   #198112

   Purpose

   lentiviral expression of hCOL4A4_C1684X in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Lentiviral

   Tags

   Nluc

   Expression

   Mutation

   C1684X

   Promoter

   EF1a

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-Puro_CI-hCOL4A5-Nluc_C29X

   Plasmid

   #198107

   Purpose

   lentiviral expression of hCOL4A5_C29X in mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Lentiviral

   Tags

   Nluc

   Expression

   Mutation

   C29X

   Promoter

   EF1a

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Puro_CI-hCOL4A5-Nluc_R1563X

   Plasmid

   #198109

   Purpose

   lentiviral expression of hCOL4A4_R1563Xin mammalian cells

   Depositor

   Jeffrey Miner

   Article

   Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

   Insert

   COL4A5 (COL4A5 Human)
   

   Use

   Lentiviral

   Tags

   Nluc

   Expression

   Mutation

   R1563X

   Promoter

   EF1a

   Available since

   March 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Puro_CI-hCOL4A5-Nluc_S36X

    Plasmid

    #198108

    Purpose

    lentiviral expression of hCOL4A4_S36X in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    S36X

    Promoter

    EF1a

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-Puro_CI-hCOL4A5-Nluc

    Plasmid

    #198106

    Purpose

    lentiviral expression of hCOL4A4 in mammalian cells

    Depositor

    Jeffrey Miner

    Article

    Omachi et al iScience. 2022 Feb 8;25(3):103891. doi:

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    Nluc

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. L1-AA027

    Plasmid

    #185752

    Purpose

    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    HygR2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCh-1478

    Plasmid

    #82424

    Purpose

    Plasmid for expression of GST-tagged Nematostella vectensis GW182 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvGW182
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMCh-1547

    Plasmid

    #82434

    Purpose

    Plasmid for expression of HA-tagged Nematostella vectensis AGO1 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvAGO1
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMCh-1548

    Plasmid

    #82435

    Purpose

    Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

    Depositor

    Marina Chekulaeva

    Article

    Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

    Insert

    nvAGO2
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-GAL9

    Plasmid

    #166689

    Purpose

    For creating a stable mCherry-GAL9 cell line

    Depositor

    Alan Sabirsh

    Article

    Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

    Insert

    LGALS9 (LGALS9 Human)
    

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132400

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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