We narrowed to 8,512 results for: sgrna

1. pC368: pAAV.CMV-Cas13e-VEGFA sgRNA2

   Plasmid

   #227800

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA2

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 2 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC369: pAAV.CMV-Cas13e-VEGFA sgRNA3

   Plasmid

   #227801

   Purpose

   Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA3

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Inserts

   U6-VEGFA sgRNA 3 (Vegfa Synthetic)
   Cas13e
   

   Use

   AAV

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

   Plasmid

   #206178

   Purpose

   Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

   Depositor

   Yuxuan Guo

   Article

   Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   cTnT

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-U6.3-sgRNA-empty-GFP

   Plasmid

   #221843

   Purpose

   empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

   Depositor

   Stephen Terry

   Article

   O’Sullivan et al bioRxiv 2024.03.04.583298

   Insert

   EGFP
   

   Use

   CRISPR; Tol2 transposon optimised for chick expre…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGC

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-U6.3-sgRNA-DNM1L -GFP

   Plasmid

   #221845

   Purpose

   Tol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoter

   Depositor

   Stephen Terry

   Article

   O’Sullivan et al bioRxiv 2024.03.04.583298

   Inserts

   EGFP
   DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
   

   Use

   CRISPR; Tol2 transposon optimised for chick expre…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGC and U6.3 chick

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-U6.3-sgRNA-MFN1-GFP

   Plasmid

   #221846

   Purpose

   Tol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promote

   Depositor

   Stephen Terry

   Article

   O’Sullivan et al bioRxiv 2024.03.04.583298

   Inserts

   EGFP
   MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
   

   Use

   CRISPR; Tol2 transposon optimised for chick expre…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGC and U6.3 chick

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-eSpCas9-2A-GFP-sgRNA-Lig4

   Plasmid

   #220494

   Purpose

   To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3.

   Depositor

   Sébastien Britton

   Article

   Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

   Insert

   sgRNA targeting LIG4 exon 3 (LIG4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF821-recipient_U6-sgRNA-EF1a-mNeonGreen

   Plasmid

   #211651

   Purpose

   U6-sgRNA-EF1a-mNeonGreen

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   Guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF820-recipient_U6-sgRNA-EF1a-mCherry2

   Plasmid

   #211647

   Purpose

   U6-sgRNA-EF1a-mCherry2

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   Guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-sgRNA-Cre-GpNLuc-sgmATM

    Plasmid

    #208392

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ATM gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgATM (Atm Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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