Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 32,840 results for: Eng

Showing: 3721 - 3750 of 32840 results

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pHAGE-EGFR-R521K

Plasmid

#116296

Purpose

Lentiviral expression of EGFR R521K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

R521K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-L245F

Plasmid

#116172

Purpose

Lentiviral expression of BRAF L245F

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

L245F

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-K499N

Plasmid

#116167

Purpose

Lentiviral expression of BRAF K499N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

K499N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-E726K

Plasmid

#116488

Purpose

Lentiviral expression of PIK3CA E726K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

E726K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-P104L

Plasmid

#116519

Purpose

Lentiviral expression of PIK3CA P104L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

P104L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-V765G

Plasmid

#116315

Purpose

Lentiviral expression of EGFR V765G

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

V765G

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-V843I

Plasmid

#116317

Purpose

Lentiviral expression of EGFR V843I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

V843I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR4-K157N

Plasmid

#116391

Purpose

Lentiviral expression of FGFR4 K157N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR4 (FGFR4 Human)

Use

Lentiviral

Mutation

K157N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR4-M708I

Plasmid

#116392

Purpose

Lentiviral expression of FGFR4 M708I

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR4 (FGFR4 Human)

Use

Lentiviral

Mutation

M708I

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR4-P788L

Plasmid

#116393

Purpose

Lentiviral expression of FGFR4 P788L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR4 (FGFR4 Human)

Use

Lentiviral

Mutation

P788L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600R

Plasmid

#116209

Purpose

Lentiviral expression of BRAF V600R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600R

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-P546S

Plasmid

#116282

Purpose

Lentiviral expression of EGFR P546S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

P546S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-A598_T599insP

Plasmid

#116131

Purpose

Lentiviral expression of BRAF A598_T599insP

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

A598_T599insP

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-P596S

Plasmid

#116285

Purpose

Lentiviral expression of EGFR P596S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

P596S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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rTTA-gRNA-AAV

Plasmid

#213036

Purpose

This vector contains rTTA expressed under CMV promoter and gRNA expressed under U6 promoter

Depositor

Nadav Ahituv

Article

Nguyen et al Nat Biotechnol. 2025 Feb 4:10.1038/s4158

Insert

rTTA-T2A-mCherry

Use

AAV and CRISPR

Promoter

CMV

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1 C122E

Plasmid

#232274

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

changed cysteine 122 to glutamic acid

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

Plasmid

#228787

Purpose

EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
EGFP***
TEV protease

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#2

Plasmid

#174147

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgAMBRA1#1

Plasmid

#174146

Purpose

Lentiviral vector expressing a sgRNA against the human AMBRA1 gene

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA (AMBRA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Δ1-60MEK1C222

Plasmid

#164641

Purpose

Bacterial expression plasmid for His6-ΔMEK1C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

S222C/C277S/C376S; residues 1-60 at N-terminus we…

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Δ1-60MEK1C218

Plasmid

#164643

Purpose

Bacterial expression plasmid for His6-ΔMEK1C218

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

S218C/C277S/C376S; residues 1-60 at N-terminus we…

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Δ1-60MEK1C218C222

Plasmid

#164645

Purpose

Bacterial expression plasmid for His6-ΔMEK1C218C222

Depositor

Ben Davis

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)

Tags

His6-tag

Expression

Bacterial

Mutation

S218C/S222C/C277S/C376S; residues 1-60 at N-termi…

Promoter

T7

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-BRAF-A404Cfs*9

Plasmid

#116129

Purpose

Lentiviral expression of BRAF A404Cfs*9

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

A404Cfs*9

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFB1-S192*

Plasmid

#116697

Purpose

Lentiviral expression of TGFB1 S192*

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Mutation

S192*

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFB1-V367M

Plasmid

#116698

Purpose

Lentiviral expression of TGFB1 V367M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Mutation

V367M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFBR2-G43C

Plasmid

#116699

Purpose

Lentiviral expression of TGFBR2 G43C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Mutation

G43C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TGFBR2-I218V

Plasmid

#116700

Purpose

Lentiviral expression of TGFBR2 I218V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Mutation

I218V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TGFBR2-R190H

Plasmid

#116701

Purpose

Lentiviral expression of TGFBR2 R190H

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Mutation

R190H

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TGFBR2-V376M

Plasmid

#116703

Purpose

Lentiviral expression of TGFBR2 V376M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Mutation

V376M

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TGFB1-G202E

Plasmid

#116695

Purpose

Lentiviral expression of TGFB1 G202E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Mutation

G202E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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