We narrowed to 18,514 results for: Jun
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Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
Tags3xMS2ExpressionMammalianPromoterSV40Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIND-GLucZ
Plasmid#228704PurposeInducible expression of Gaussia luciferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable SinceJan. 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
Tags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLIX401 APE1 SO
Plasmid#227192PurposeInducible lentiviral expression of SO (Slow Off) APE1DepositorInsertapurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
ExpressionBacterial and MammalianMutationD283A D308AAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pBa.KIF13A
Plasmid#224408PurposeMammalian expression of Kif 13aDepositorAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.Mark2-3myc
Plasmid#224416PurposeMammalian expression of MARK2DepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspB
Plasmid#215400PurposeCm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)DepositorInsertcspB
Tags6xHis TagExpressionBacterialPromoterT7 promoterAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA2
Plasmid#215399PurposeCm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)DepositorInsertcspA2
Tags6xHis TagExpressionBacterialPromoterT7 promoterAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PLCd1(PHD)
Plasmid#221753PurposeStable expression of GFP-PLCd1(PHD) in mammalian cellsDepositorAvailable SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(Y557C)
Plasmid#209695PurposeExpresses MAC3-tagged CCDC22(Y557C) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
TagsMAC3-tagExpressionMammalianMutationY557C, changed Tyrosine 557 to CysteineAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(T17A)
Plasmid#209694PurposeExpresses MAC3-tagged CCDC22(T17A) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
TagsMAC3-tagExpressionMammalianMutationT17A, changed Threonine 17 to AlanineAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-PES-H1-L92F-Q94Y
Plasmid#218705PurposeEncodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expressionDepositorInsertPES-H1
Tags10xHisExpressionBacterialMutationL92F-Q94YAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX_K1033R
Plasmid#221378PurposeExpresses ATRX_K1033R in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
ExpressionBacterialMutationChanged lysine 1033 to argininePromotertacAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM_K819R
Plasmid#221380PurposeExpresses FANCM_K819R in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
ExpressionBacterialMutationChanged lysine 819 to argininePromotertacAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEYFP_C1_ATRX
Plasmid#221383PurposeExpresses ATRX in mammalian cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
ExpressionMammalianMutationK536N (Please see depositor comments)PromoterCMVAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSK519-cspB
Plasmid#207162PurposeExpresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vectorDepositorInsertcspB
TagsHAExpressionBacterialPromoternptIIAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-10xHis-ecRNHI*
Plasmid#219523Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI geneDepositorInsertcysteine-free variant of the E. coli ribonuclease HI gene
TagsN terminal 10xHis TagExpressionBacterialMutationCysteine free variantAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-TfCut2-L32E-S113E-T237Q
Plasmid#218696PurposeEncodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expressionDepositorInsertTfCut2
Tags10xHisExpressionBacterialMutationL32E-S113E-T237QAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
Plasmid#216403PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/Q443P
Tags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 70, 443 to P…Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianPromoterCMVAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
IAA17-4flag-HIS5
Plasmid#207015PurposeFor ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-HIS5
Plasmid#207014PurposePlasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-MET15
Plasmid#207013PurposePlasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-URA3
Plasmid#207012PurposePlasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10D(E215Q)-HA
Plasmid#209260PurposeMammalian expression of ATP10D mutant E215QDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10B(E210Q)-HA
Plasmid#209247PurposeMammalian expression of ATP10B mutant E210QDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10B-HA
Plasmid#209233PurposeMammalian expression of ATP10BDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10B(E210Q)-HA
Plasmid#209259PurposeMammalian expression of ATP10B mutant E210QDepositorAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10A-HA
Plasmid#209232PurposeMammalian expression of ATP10ADepositorAvailable SinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-PHLPP2
Plasmid#203605PurposeReporter plasmid carrying PHLPP2 3'UTRDepositorInsertPH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)
UseLuciferaseExpressionMammalianPromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-27bT
Plasmid#203600PurposeReporter plasmid carrying miR-27b target sequenceDepositorInsertcomplementary sequence to miR27b-3p (MIR27B Human)
UseLuciferaseExpressionMammalianPromoterSV40Available SinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
Plasmid#201534PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB3
UseSynthetic BiologyMutationrhaS W190GPromoterPrhaBAD (rhamnose)Available SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVA4_VP1
Plasmid#202068PurposeBacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVA4 VP1 protein with Histag
Tags6xHis-tag and GSTExpressionBacterialPromotertacAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2
Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-domain
Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
TagsChiting Binding Domanin-InteinExpressionBacterialPromoterT7Available SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- BAD-YFP
Plasmid#186567PurposepMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
ExpressionBacterialPromoterBAD promoterAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-delM2_H
Plasmid#146521PurposeInsect Expression of DmGW182-delM2DepositorInsertDmGW182-delM2 (gw Fly)
ExpressionInsectMutationTwo non silent mutations V704A and N799S and one …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6A_1-1248_H
Plasmid#146507PurposeMammalian Expression of HsTNRC6A_1-1248DepositorInsertHsTNRC6A_1-1248 (TNRC6A Human)
ExpressionMammalianMutation254-1962 truncated version of the sequence given …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-dsRNAres_G
Plasmid#146431PurposeInsect Expression of DmGW182-dsRNAresDepositorInsertDmGW182-dsRNAres (gw Fly)
ExpressionInsectMutationtwo non silent mutations V704A and N799S, and one…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-del-M-GW-Cterm_F
Plasmid#146324PurposeInsect Expression of DmGW182-del-M-GW-CtermDepositorInsertDmGW182-del-M-GW-Cterm (gw Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-12xmut_F
Plasmid#146320PurposeInsect Expression of DmGW182-12xmutDepositorInsertDmGW182-12xmut (gw Fly)
ExpressionInsectMutationTwo non silent mutations V704A and N799S and one …Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only