We narrowed to 3,637 results for: golden gate

1. JD81

   Plasmid

   #229481

   Purpose

   Adenoviral VA RNA II gene

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   VA RNA II
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   wt promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JD92

   Plasmid

   #229478

   Purpose

   CMV promoter driving expression of adenoviral E4orf6 gene

   Depositor

   Nerea Zabaleta

   Article

   Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

   Insert

   E4orf6
   

   Use

   AAV and Adenoviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGC018

   Plasmid

   #228232

   Purpose

   Level 0 for LhG4 (GreenGate C module)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   GR-LhG4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   -

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUS-miRFP670-Coilin

   Plasmid

   #227386

   Purpose

   Adhesion module, creates 'seed' at Cajal body

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Cajal body FUS IDR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgTelo

   Plasmid

   #227392

   Purpose

   Adhesion module, guide RNA recognizes telomeres

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   guide RNA targeting telomeres
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgPPP1R2

   Plasmid

   #227391

   Purpose

   Adhesion module, guide RNA recognizes PPP1R2

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   guide RNA targeting PPP1R2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB68

   Plasmid

   #226315

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJB51

   Plasmid

   #226308

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with V(T/S)G to AAA CsoS2 MR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   VTG273-275AAA, VTG284-286AAA, VTG295-297AAA

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB48

   Plasmid

   #226307

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with C to S CsoS2 MR1-6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

   Promoter

   pTRC

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJB47

    Plasmid

    #226306

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S, C526S, …

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJB46

    Plasmid

    #226305

    Purpose

    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    CsoS2 with C to S CsoS2 MR1-4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C292S, C310S, C351S, C369S, C467S, C485S

    Promoter

    pTRC

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJJB170

    Plasmid

    #218591

    Purpose

    Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Adr1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJB1340

    Plasmid

    #218593

    Purpose

    Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex22
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Pex22(1-36)

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSKT001

    Plasmid

    #218594

    Purpose

    Express degron assay: Degradation tag-YFP-ePTS1

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    YFP
    

    Use

    Tags

    UbiY degradation tag and ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJJB1357

    Plasmid

    #218595

    Purpose

    Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex11
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKS1461

    Plasmid

    #218587

    Purpose

    Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Erg12
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TC alphaDdCBE T2

    Plasmid

    #221991

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TC TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ND2 alphaDdCBE T2

    Plasmid

    #221977

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-ND2 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TC mDdCBE A1

    Plasmid

    #221986

    Purpose

    Expresses monomeric mitochondrial cytosine base editor in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TC TALE A1 repeats-TALE CTD-DddAtox GSVG-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1326G, G1348S, A1398V, S1418G

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TC mDdCBE T2

    Plasmid

    #221987

    Purpose

    Expresses monomeric mitochondrial cytosine base editor in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TC TALE T2 repeats-TALE CTD-DddAtox GSVG-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: S1326G, G1348S, A1398V, S1418G

    Promoter

    CMV

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge