We narrowed to 69,454 results for: nin

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pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-NucGFP

Plasmid

#184696

Purpose

Lentiviral transfer plasmid for expression of NucGFP

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

NucGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAtipiTrc

Plasmid

#53275

Purpose

Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

Insert

ipi1 (IPP2 Mustard Weed)

Expression

Bacterial

Promoter

Trc

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUM31dao

Plasmid

#87242

Purpose

Plastid expression cassette containing Schizosaccharomyces pombe D-amino acid oxidase gene. Provides dual positive (D-alanine) and negative (D-valine) selection in transplastomic plants

Depositor

Anil Day

Article

Gisby et al Plant Physiol. 2012 Dec;160(4):2219-26.

Insert

D-amino oxidase

Expression

Plant

Promoter

plastid rrn

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen

Plasmid

#184695

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-AILR-LplA-ER

Plasmid

#42574

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LplA(AILR) with ER-retention sequence KDEL

Tags

FLAG

Mutation

Compared to wild-type LplA: tryptophan 37 to alan…

Promoter

CAG

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
fADL-1e-flag-linker-p3

Plasmid

#139441

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, flag-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Myog'-2A-mCherryNLS-PGK-Purodtk

Plasmid

#69547

Purpose

Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Inserts

2A-mCherry-NLS
Myogenin 5' homology arm (Myog Mouse)
Myogenin 3' homology arm (Myog Mouse)
Puro-delta-tk

Use

Mouse Targeting

Tags

2x nuclear localization signal and PVT1-2A "…

Mutation

Silent V205V mutation in myogenin gene to prevent…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-FRB-HA no-pA

Plasmid

#82594

Purpose

3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDYSCKO

Plasmid

#120263

Purpose

Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

Depositor

Christian Landry

Article

Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

Insert

DYSCKO cassette

Use

CRISPR and Synthetic Biology

Promoter

SNR52

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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S3_002_026_096

Plasmid

#134403

Purpose

pYTK096-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N

Tags

None and none

Expression

Yeast

Mutation

None and none

Promoter

pGPM1 and pRPL18B

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDY4

Plasmid

#53589

Purpose

Produces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Proc Natl Acad Sci U S A. 2001 Feb 27;98

Insert

lcyE

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pME-BTK-PH

Plasmid

#67673

Purpose

Gateway entry clone containing BTK-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

BTK-PH-NS

Use

Gateway multisite middle entry clone

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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S3_004_026_MV1

Plasmid

#134416

Purpose

pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pGBT9-chNfasc (+RSLE)

Plasmid

#89418

Purpose

Two-hybrid system

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

NFASC (NFASC Chicken)

Tags

GAL4 DBD

Expression

Yeast

Promoter

ADH1

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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